Submicron-precision particle characterization in microfluidic impedance cytometry with double differential electrodes
Submicron-precision particle characterization is crucial for counting, sizing and identifying a variety of biological particles, such as bacteria and apoptotic bodies. Microfluidic impedance cytometry has been attractive in current research for microparticle characterization due to its advantages of label-free detection, ease of miniaturization and affordability. However, conventional electrode configurations of three electrodes and floating electrodes have not yet demonstrated the capability of probing submicron particles or microparticles with a submicron size difference. In this study, we present a label-free high-throughput (∼800 particles per second) impedance-based microfluidic flow cytometry system integrated with a novel design of a double differential electrode configuration, enabling submicron particle detection (down to 0.4 μm) with a minimum size resolution of 200 nm. The signal-to-noise ratio has been boosted from 13.98 dB to 32.64 dB compared to a typical three-electrode configuration. With the proposed microfluidic impedance cytometry, we have shown results of sizing microparticles that accurately correlate with manufacturers' datasheets (R2 = 0.99938). It also shows that population ratios of differently sized beads in mixture samples are consistent with the results given by commercial fluorescence-based flow cytometry (within ∼1% difference). This work provides a label-free approach with submicron precision for sizing and counting microscale and submicron particles, and a new avenue of designing electrode configurations with a feature of suppressing the electrical noise for accomplishing a high signal-to-noise ratio in a wide range of frequencies. This novel double differential impedance sensing system paves a new pathway for real-time analysis and accurate particle screening in pathological and pharmacological research.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:21 |
---|---|
Enthalten in: |
Lab on a chip - 21(2021), 15 vom: 07. Aug., Seite 2869-2880 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Zhong, Jianwei [VerfasserIn] |
---|
Links: |
---|
Themen: |
---|
Anmerkungen: |
Date Completed 30.07.2021 Date Revised 30.07.2021 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1039/d1lc00481f |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM327820403 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM327820403 | ||
003 | DE-627 | ||
005 | 20231225201629.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1039/d1lc00481f |2 doi | |
028 | 5 | 2 | |a pubmed24n1092.xml |
035 | |a (DE-627)NLM327820403 | ||
035 | |a (NLM)34236057 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Zhong, Jianwei |e verfasserin |4 aut | |
245 | 1 | 0 | |a Submicron-precision particle characterization in microfluidic impedance cytometry with double differential electrodes |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 30.07.2021 | ||
500 | |a Date Revised 30.07.2021 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Submicron-precision particle characterization is crucial for counting, sizing and identifying a variety of biological particles, such as bacteria and apoptotic bodies. Microfluidic impedance cytometry has been attractive in current research for microparticle characterization due to its advantages of label-free detection, ease of miniaturization and affordability. However, conventional electrode configurations of three electrodes and floating electrodes have not yet demonstrated the capability of probing submicron particles or microparticles with a submicron size difference. In this study, we present a label-free high-throughput (∼800 particles per second) impedance-based microfluidic flow cytometry system integrated with a novel design of a double differential electrode configuration, enabling submicron particle detection (down to 0.4 μm) with a minimum size resolution of 200 nm. The signal-to-noise ratio has been boosted from 13.98 dB to 32.64 dB compared to a typical three-electrode configuration. With the proposed microfluidic impedance cytometry, we have shown results of sizing microparticles that accurately correlate with manufacturers' datasheets (R2 = 0.99938). It also shows that population ratios of differently sized beads in mixture samples are consistent with the results given by commercial fluorescence-based flow cytometry (within ∼1% difference). This work provides a label-free approach with submicron precision for sizing and counting microscale and submicron particles, and a new avenue of designing electrode configurations with a feature of suppressing the electrical noise for accomplishing a high signal-to-noise ratio in a wide range of frequencies. This novel double differential impedance sensing system paves a new pathway for real-time analysis and accurate particle screening in pathological and pharmacological research | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
700 | 1 | |a Liang, Minhui |e verfasserin |4 aut | |
700 | 1 | |a Ai, Ye |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Lab on a chip |d 2001 |g 21(2021), 15 vom: 07. Aug., Seite 2869-2880 |w (DE-627)NLM147083753 |x 1473-0189 |7 nnns |
773 | 1 | 8 | |g volume:21 |g year:2021 |g number:15 |g day:07 |g month:08 |g pages:2869-2880 |
856 | 4 | 0 | |u http://dx.doi.org/10.1039/d1lc00481f |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 21 |j 2021 |e 15 |b 07 |c 08 |h 2869-2880 |