Injectable gelatin microspheres loaded with platelet rich plasma improve wound healing by regulating early inflammation
© The author(s)..
We investigated the potential of gelatin microspheres (GMs) loaded with platelet-rich plasma (PRP) to enhance their wound healing effect. Platelets from the PRP were immobilized onto GMs to form biomimetic bioreactor GM+PRP. The therapeutic effect of this agent was further investigated in vivo on a wound-healing model in rats. Wounds were locally injected with phosphate buffered saline (PBS), GM, PRP, and GM+PRP. Wound healing rate, vessel density, and inflammation level were measured histologically, by RT-PCR, and by Western blotting at days 3, 7, 14, and 21. Platelets on GM caused a continuous high release in both interleukin-10 and metalloproteinase-3 compared with PRP alone. Both GM+PRP and PRP successfully accelerated the wound healing process, while GM alone did not improve the wound healing process compared with the untreated control. Wounds treated with GM+PRP resulted in shorter healing period and improved dermal structure. GM+PRP improved angiogenesis in the wound by increasing expression of angiogenic factors. GM+PRP prolonged and enhanced the cytokine release profile compared with PRP. By promoting the inflammatory and angiogenic responses, GM+PRP has the potential to improve wound healing. Our findings demonstrate that GMs are an injectable carrier that enhanced the therapeutic effects of PRP.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
International journal of medical sciences - 18(2021), 9 vom: 25., Seite 1910-1920 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhou, Shaolong [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.11.2021 Date Revised 11.11.2023 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.7150/ijms.51060 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Injectable gelatin microspheres loaded with platelet rich plasma improve wound healing by regulating early inflammation |
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| 520 | |a © The author(s). | ||
| 520 | |a We investigated the potential of gelatin microspheres (GMs) loaded with platelet-rich plasma (PRP) to enhance their wound healing effect. Platelets from the PRP were immobilized onto GMs to form biomimetic bioreactor GM+PRP. The therapeutic effect of this agent was further investigated in vivo on a wound-healing model in rats. Wounds were locally injected with phosphate buffered saline (PBS), GM, PRP, and GM+PRP. Wound healing rate, vessel density, and inflammation level were measured histologically, by RT-PCR, and by Western blotting at days 3, 7, 14, and 21. Platelets on GM caused a continuous high release in both interleukin-10 and metalloproteinase-3 compared with PRP alone. Both GM+PRP and PRP successfully accelerated the wound healing process, while GM alone did not improve the wound healing process compared with the untreated control. Wounds treated with GM+PRP resulted in shorter healing period and improved dermal structure. GM+PRP improved angiogenesis in the wound by increasing expression of angiogenic factors. GM+PRP prolonged and enhanced the cytokine release profile compared with PRP. By promoting the inflammatory and angiogenic responses, GM+PRP has the potential to improve wound healing. Our findings demonstrate that GMs are an injectable carrier that enhanced the therapeutic effects of PRP | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a early inflammation | |
| 650 | 4 | |a gelatin microspheres | |
| 650 | 4 | |a platelet-rich plasma | |
| 650 | 4 | |a tissue regeneration | |
| 650 | 4 | |a wound healing | |
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| 700 | 1 | |a Chen, Chen |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Linhua |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Fiona H |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Jianghui |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Liping |e verfasserin |4 aut | |
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