Interaction standards for biophysics : anti-lysozyme nanobodies
There is a significant demand in the molecular biophysics community for robust standard samples. They are required by researchers, instrument developers and pharmaceutical companies for instrumental quality control, methodological development and in the design and validation of devices, diagnostics and instrumentation. To-date there has been no clear consensus on the need and type of standards that should be available and different research groups and instrument manufacturers use different standard systems which significantly hinders comparative analysis. One of the major objectives of the Association of Resources for Biophysical Research in Europe (ARBRE) is to establish a common set of standard samples that can be used throughout the biophysics community and instrument developers. A survey was circulated among ARBRE members to ascertain the requirements of laboratories when using standard systems and the results are documented in this article. In summary, the major requirements are protein samples which are cheap, relatively small, stable and have different binding strengths. We have developed a panel of sdAb's or 'nanobodies' against hen-egg white lysozyme with different binding strengths and suitable stability characteristics. Here we show the results of the survey, the selection procedure, validation and final selection of a panel of nanobody interaction standards.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:50 |
|---|---|
| Enthalten in: |
European biophysics journal : EBJ - 50(2021), 3-4 vom: 10. Mai, Seite 333-343 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Birchenough, Holly L [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
EC 3.2.1.17 |
|---|
| Anmerkungen: |
Date Completed 29.11.2021 Date Revised 29.11.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1007/s00249-021-01524-6 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM323949487 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM323949487 | ||
| 003 | DE-627 | ||
| 005 | 20231225185256.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s00249-021-01524-6 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1079.xml |
| 035 | |a (DE-627)NLM323949487 | ||
| 035 | |a (NLM)33839878 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Birchenough, Holly L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Interaction standards for biophysics |b anti-lysozyme nanobodies |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 29.11.2021 | ||
| 500 | |a Date Revised 29.11.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a There is a significant demand in the molecular biophysics community for robust standard samples. They are required by researchers, instrument developers and pharmaceutical companies for instrumental quality control, methodological development and in the design and validation of devices, diagnostics and instrumentation. To-date there has been no clear consensus on the need and type of standards that should be available and different research groups and instrument manufacturers use different standard systems which significantly hinders comparative analysis. One of the major objectives of the Association of Resources for Biophysical Research in Europe (ARBRE) is to establish a common set of standard samples that can be used throughout the biophysics community and instrument developers. A survey was circulated among ARBRE members to ascertain the requirements of laboratories when using standard systems and the results are documented in this article. In summary, the major requirements are protein samples which are cheap, relatively small, stable and have different binding strengths. We have developed a panel of sdAb's or 'nanobodies' against hen-egg white lysozyme with different binding strengths and suitable stability characteristics. Here we show the results of the survey, the selection procedure, validation and final selection of a panel of nanobody interaction standards | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Interaction technologies | |
| 650 | 4 | |a Molecular standards | |
| 650 | 4 | |a Nanobodies | |
| 650 | 4 | |a Phage display | |
| 650 | 4 | |a Single domain antibodies | |
| 650 | 7 | |a Single-Domain Antibodies |2 NLM | |
| 650 | 7 | |a Muramidase |2 NLM | |
| 650 | 7 | |a EC 3.2.1.17 |2 NLM | |
| 700 | 1 | |a Nivia, Hilda D Ruiz |e verfasserin |4 aut | |
| 700 | 1 | |a Jowitt, Thomas A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t European biophysics journal : EBJ |d 1991 |g 50(2021), 3-4 vom: 10. Mai, Seite 333-343 |w (DE-627)NLM012633011 |x 1432-1017 |7 nnns |
| 773 | 1 | 8 | |g volume:50 |g year:2021 |g number:3-4 |g day:10 |g month:05 |g pages:333-343 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00249-021-01524-6 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 50 |j 2021 |e 3-4 |b 10 |c 05 |h 333-343 | ||