Reprogramming progressive cells display low CAG promoter activity
© 2020 The Authors. STEM CELLS published by Wiley Periodicals LLC on behalf of AlphaMed Press..
There is wide variability in the propensity of somatic cells to reprogram into pluripotency in response to the Yamanaka factors. How to segregate these variabilities to enrich for cells of specific traits that reprogram efficiently remains challenging. Here we report that the variability in reprogramming propensity is associated with the activity of the MKL1/SRF transcription factor and concurs with small cell size as well as rapid cell cycle. Reprogramming progressive cells can be prospectively identified by their low activity of a widely used synthetic promoter, CAG. CAGlow cells arise and expand during cell cycle acceleration in the early reprogramming culture of both mouse and human fibroblasts. Our work illustrates a molecular scenario underlying the distinct reprogramming propensities and demonstrates a convenient practical approach for their enrichment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:39 |
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| Enthalten in: |
Stem cells (Dayton, Ohio) - 39(2021), 1 vom: 25. Jan., Seite 43-54 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hu, Xiao [VerfasserIn] |
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| Links: |
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| Themen: |
CAG promoter |
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| Anmerkungen: |
Date Completed 06.12.2021 Date Revised 22.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/stem.3295 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM316445428 |
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| 520 | |a © 2020 The Authors. STEM CELLS published by Wiley Periodicals LLC on behalf of AlphaMed Press. | ||
| 520 | |a There is wide variability in the propensity of somatic cells to reprogram into pluripotency in response to the Yamanaka factors. How to segregate these variabilities to enrich for cells of specific traits that reprogram efficiently remains challenging. Here we report that the variability in reprogramming propensity is associated with the activity of the MKL1/SRF transcription factor and concurs with small cell size as well as rapid cell cycle. Reprogramming progressive cells can be prospectively identified by their low activity of a widely used synthetic promoter, CAG. CAGlow cells arise and expand during cell cycle acceleration in the early reprogramming culture of both mouse and human fibroblasts. Our work illustrates a molecular scenario underlying the distinct reprogramming propensities and demonstrates a convenient practical approach for their enrichment | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Yamanaka reprogramming | |
| 650 | 4 | |a cell cycle speed | |
| 650 | 4 | |a cell size | |
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| 700 | 1 | |a Wu, Qiao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Jonghun |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xinyue |e verfasserin |4 aut | |
| 700 | 1 | |a Hartman, Amaleah A |e verfasserin |4 aut | |
| 700 | 1 | |a Eastman, Anna E |e verfasserin |4 aut | |
| 700 | 1 | |a Park, In-Hyun |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Shangqin |e verfasserin |4 aut | |
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