Oxidative damage mechanism in Saccharomyces cerevisiae cells exposed to tetrachlorobisphenol A
Copyright © 2020 Elsevier B.V. All rights reserved..
Tetrachlorobisphenol A (TCBPA) can promote intracellular reactive oxygen species (ROS) accumulation. However, limited attention has been given to mechanisms underlying TCBPA exposure-associated ROS accumulation. Here, such mechanisms were explored in the simple eukaryotic model organism Saccharomyces cerevisiae exposed to multiple concentrations of TCBPA. Addition of diphenyleneiodonium, a specific inhibitor of NADPH oxidase, blocked TCBPA treatment-associated intracellular ROS accumulation. NADPH oxidase can be activated by calcineurin, mitogen-activated protein kinase (MAPK), and tyrosine kinase. Therefore, corresponding specific inhibition respectively on these three kinases was performed and results suggested that the Ca2+ signaling pathway, MAPK pathway, and tyrosine kinase pathway all contributed to the TCBPA exposure-associated intracellular ROS accumulation. In addition, TCBPA exposure-associated up-regulation of genes involved in ROS production and down-regulation of catalase promoted ROS accumulation in S. cerevisiae. To sum up, our current results provide insights into the understanding of TCBPA exposure-associated ROS accumulation.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2020 |
---|---|
Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:80 |
---|---|
Enthalten in: |
Environmental toxicology and pharmacology - 80(2020) vom: 01. Nov., Seite 103507 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Zhang, Xiaoru [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 03.02.2021 Date Revised 03.02.2021 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1016/j.etap.2020.103507 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM315778075 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM315778075 | ||
003 | DE-627 | ||
005 | 20231225155642.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2020 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.etap.2020.103507 |2 doi | |
028 | 5 | 2 | |a pubmed24n1052.xml |
035 | |a (DE-627)NLM315778075 | ||
035 | |a (NLM)33007436 | ||
035 | |a (PII)S1382-6689(20)30183-6 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Zhang, Xiaoru |e verfasserin |4 aut | |
245 | 1 | 0 | |a Oxidative damage mechanism in Saccharomyces cerevisiae cells exposed to tetrachlorobisphenol A |
264 | 1 | |c 2020 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 03.02.2021 | ||
500 | |a Date Revised 03.02.2021 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
520 | |a Tetrachlorobisphenol A (TCBPA) can promote intracellular reactive oxygen species (ROS) accumulation. However, limited attention has been given to mechanisms underlying TCBPA exposure-associated ROS accumulation. Here, such mechanisms were explored in the simple eukaryotic model organism Saccharomyces cerevisiae exposed to multiple concentrations of TCBPA. Addition of diphenyleneiodonium, a specific inhibitor of NADPH oxidase, blocked TCBPA treatment-associated intracellular ROS accumulation. NADPH oxidase can be activated by calcineurin, mitogen-activated protein kinase (MAPK), and tyrosine kinase. Therefore, corresponding specific inhibition respectively on these three kinases was performed and results suggested that the Ca2+ signaling pathway, MAPK pathway, and tyrosine kinase pathway all contributed to the TCBPA exposure-associated intracellular ROS accumulation. In addition, TCBPA exposure-associated up-regulation of genes involved in ROS production and down-regulation of catalase promoted ROS accumulation in S. cerevisiae. To sum up, our current results provide insights into the understanding of TCBPA exposure-associated ROS accumulation | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Reactive oxygen species accumulation | |
650 | 4 | |a Saccharomyces cerevisiae | |
650 | 4 | |a Signaling pathway | |
650 | 4 | |a Tetrachlorobisphenol A | |
650 | 7 | |a Chlorophenols |2 NLM | |
650 | 7 | |a Flame Retardants |2 NLM | |
650 | 7 | |a Onium Compounds |2 NLM | |
650 | 7 | |a Reactive Oxygen Species |2 NLM | |
650 | 7 | |a Saccharomyces cerevisiae Proteins |2 NLM | |
650 | 7 | |a diphenyleneiodonium |2 NLM | |
650 | 7 | |a 6HJ411TU98 |2 NLM | |
650 | 7 | |a CTA1 protein, S cerevisiae |2 NLM | |
650 | 7 | |a EC 1.11.1.6 |2 NLM | |
650 | 7 | |a Catalase |2 NLM | |
650 | 7 | |a EC 1.11.1.6 |2 NLM | |
650 | 7 | |a Superoxide Dismutase-1 |2 NLM | |
650 | 7 | |a EC 1.15.1.1 |2 NLM | |
650 | 7 | |a NADPH Oxidases |2 NLM | |
650 | 7 | |a EC 1.6.3.- |2 NLM | |
650 | 7 | |a Mitogen-Activated Protein Kinases |2 NLM | |
650 | 7 | |a EC 2.7.11.24 |2 NLM | |
650 | 7 | |a tetrachlorodian |2 NLM | |
650 | 7 | |a FO0P9ET4BN |2 NLM | |
650 | 7 | |a Calcium |2 NLM | |
650 | 7 | |a SY7Q814VUP |2 NLM | |
700 | 1 | |a Zhang, Yaxian |e verfasserin |4 aut | |
700 | 1 | |a Ji, Zhihua |e verfasserin |4 aut | |
700 | 1 | |a Wang, Fengbang |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Lei |e verfasserin |4 aut | |
700 | 1 | |a Song, Maoyong |e verfasserin |4 aut | |
700 | 1 | |a Li, Hao |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Environmental toxicology and pharmacology |d 1996 |g 80(2020) vom: 01. Nov., Seite 103507 |w (DE-627)NLM095523839 |x 1872-7077 |7 nnns |
773 | 1 | 8 | |g volume:80 |g year:2020 |g day:01 |g month:11 |g pages:103507 |
856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.etap.2020.103507 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 80 |j 2020 |b 01 |c 11 |h 103507 |