Oxidative damage mechanism in Saccharomyces cerevisiae cells exposed to tetrachlorobisphenol A
Copyright © 2020 Elsevier B.V. All rights reserved..
Tetrachlorobisphenol A (TCBPA) can promote intracellular reactive oxygen species (ROS) accumulation. However, limited attention has been given to mechanisms underlying TCBPA exposure-associated ROS accumulation. Here, such mechanisms were explored in the simple eukaryotic model organism Saccharomyces cerevisiae exposed to multiple concentrations of TCBPA. Addition of diphenyleneiodonium, a specific inhibitor of NADPH oxidase, blocked TCBPA treatment-associated intracellular ROS accumulation. NADPH oxidase can be activated by calcineurin, mitogen-activated protein kinase (MAPK), and tyrosine kinase. Therefore, corresponding specific inhibition respectively on these three kinases was performed and results suggested that the Ca2+ signaling pathway, MAPK pathway, and tyrosine kinase pathway all contributed to the TCBPA exposure-associated intracellular ROS accumulation. In addition, TCBPA exposure-associated up-regulation of genes involved in ROS production and down-regulation of catalase promoted ROS accumulation in S. cerevisiae. To sum up, our current results provide insights into the understanding of TCBPA exposure-associated ROS accumulation.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:80 |
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| Enthalten in: |
Environmental toxicology and pharmacology - 80(2020) vom: 01. Nov., Seite 103507 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Xiaoru [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 03.02.2021 Date Revised 03.02.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.etap.2020.103507 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM315778075 |
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| 245 | 1 | 0 | |a Oxidative damage mechanism in Saccharomyces cerevisiae cells exposed to tetrachlorobisphenol A |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a Tetrachlorobisphenol A (TCBPA) can promote intracellular reactive oxygen species (ROS) accumulation. However, limited attention has been given to mechanisms underlying TCBPA exposure-associated ROS accumulation. Here, such mechanisms were explored in the simple eukaryotic model organism Saccharomyces cerevisiae exposed to multiple concentrations of TCBPA. Addition of diphenyleneiodonium, a specific inhibitor of NADPH oxidase, blocked TCBPA treatment-associated intracellular ROS accumulation. NADPH oxidase can be activated by calcineurin, mitogen-activated protein kinase (MAPK), and tyrosine kinase. Therefore, corresponding specific inhibition respectively on these three kinases was performed and results suggested that the Ca2+ signaling pathway, MAPK pathway, and tyrosine kinase pathway all contributed to the TCBPA exposure-associated intracellular ROS accumulation. In addition, TCBPA exposure-associated up-regulation of genes involved in ROS production and down-regulation of catalase promoted ROS accumulation in S. cerevisiae. To sum up, our current results provide insights into the understanding of TCBPA exposure-associated ROS accumulation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Reactive oxygen species accumulation | |
| 650 | 4 | |a Saccharomyces cerevisiae | |
| 650 | 4 | |a Signaling pathway | |
| 650 | 4 | |a Tetrachlorobisphenol A | |
| 650 | 7 | |a Chlorophenols |2 NLM | |
| 650 | 7 | |a Flame Retardants |2 NLM | |
| 650 | 7 | |a Onium Compounds |2 NLM | |
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| 700 | 1 | |a Zhang, Yaxian |e verfasserin |4 aut | |
| 700 | 1 | |a Ji, Zhihua |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Fengbang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Maoyong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Hao |e verfasserin |4 aut | |
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