Isolation of Nucleus Pulposus and Annulus Fibrosus Cells from the Intervertebral Disc
Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:2221 |
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Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2221(2021) vom: 26., Seite 41-52 |
Sprache: |
Englisch |
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Beteiligte Personen: |
van den Akker, Guus G H [VerfasserIn] |
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Links: |
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Themen: |
Annulus fibrosus |
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Anmerkungen: |
Date Completed 30.03.2021 Date Revised 30.03.2021 published: Print Citation Status MEDLINE |
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doi: |
10.1007/978-1-0716-0989-7_4 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM315499214 |
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520 | |a Cells isolated from the intervertebral disc are often used for in vitro experimentation. Correctly separating the intervertebral disc tissue in annulus fibrosus and nucleus pulposus is particularly challenging when working with surplus material from surgery or specimens from donors with an advanced age. Moreover, lineage controls are only sparsely reported to verify tissue of origin. Here we describe an approach to intervertebral disc cell isolation from human and bovine origin | ||
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650 | 4 | |a Annulus fibrosus | |
650 | 4 | |a Cell characterization | |
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700 | 1 | |a Welting, Tim J M |e verfasserin |4 aut | |
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