Combinatorial Methylerythritol Phosphate Pathway Engineering and Process Optimization for Increased Menaquinone-7 Synthesis in Bacillus subtilis
Vitamin K2 (menaquinone) is an essential vitamin existing in the daily diet, and menaquinone-7 (MK-7) is an important form of it. In a recent work, we engineered the synthesis modules of MK-7 in Bacillus subtilis, and the strain BS20 could produce 360 mg/l MK-7 in shake flasks, while the methylerythritol phosphate (MEP) pathway, which provides the precursor isopentenyl diphosphate for MK-7 synthesis, was not engineered. In this study, we overexpressed five genes of the MEP pathway in BS20 and finally obtained a strain (BS20DFHG) with MK-7 titer of 415 mg/l in shake flasks. Next, we optimized the fermentation process parameters (initial pH, temperature and aeration) in an 8-unit parallel bioreactor system consisting of 300-ml glass vessels. Based on this, we scaled up the MK-7 production by the strain BS20DFHG in a 50-l bioreactor, and the highest MK-7 titer reached 242 mg/l. Here, we show that the engineered strain BS20DFHG may be used for the industrial production of MK-7 in the future.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
|---|---|
| Enthalten in: |
Journal of microbiology and biotechnology - 30(2020), 5 vom: 28. Mai, Seite 762-769 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Chen, Taichi [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
11032-49-8 |
|---|
| Anmerkungen: |
Date Completed 22.01.2021 Date Revised 29.02.2024 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.4014/jmb.1912.12008 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM310633125 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM310633125 | ||
| 003 | DE-627 | ||
| 005 | 20240229233406.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.4014/jmb.1912.12008 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1311.xml |
| 035 | |a (DE-627)NLM310633125 | ||
| 035 | |a (NLM)32482943 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Chen, Taichi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Combinatorial Methylerythritol Phosphate Pathway Engineering and Process Optimization for Increased Menaquinone-7 Synthesis in Bacillus subtilis |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 22.01.2021 | ||
| 500 | |a Date Revised 29.02.2024 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Vitamin K2 (menaquinone) is an essential vitamin existing in the daily diet, and menaquinone-7 (MK-7) is an important form of it. In a recent work, we engineered the synthesis modules of MK-7 in Bacillus subtilis, and the strain BS20 could produce 360 mg/l MK-7 in shake flasks, while the methylerythritol phosphate (MEP) pathway, which provides the precursor isopentenyl diphosphate for MK-7 synthesis, was not engineered. In this study, we overexpressed five genes of the MEP pathway in BS20 and finally obtained a strain (BS20DFHG) with MK-7 titer of 415 mg/l in shake flasks. Next, we optimized the fermentation process parameters (initial pH, temperature and aeration) in an 8-unit parallel bioreactor system consisting of 300-ml glass vessels. Based on this, we scaled up the MK-7 production by the strain BS20DFHG in a 50-l bioreactor, and the highest MK-7 titer reached 242 mg/l. Here, we show that the engineered strain BS20DFHG may be used for the industrial production of MK-7 in the future | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bacillus subtilis | |
| 650 | 4 | |a Menaquinone | |
| 650 | 4 | |a methylerythritol phosphate pathway | |
| 650 | 4 | |a process optimization | |
| 650 | 7 | |a Vitamin K 2 |2 NLM | |
| 650 | 7 | |a 11032-49-8 |2 NLM | |
| 650 | 7 | |a menaquinone 7 |2 NLM | |
| 650 | 7 | |a 8427BML8NY |2 NLM | |
| 650 | 7 | |a Erythritol |2 NLM | |
| 650 | 7 | |a RA96B954X6 |2 NLM | |
| 700 | 1 | |a Xia, Hongzhi |e verfasserin |4 aut | |
| 700 | 1 | |a Cui, Shixiu |e verfasserin |4 aut | |
| 700 | 1 | |a Lv, Xueqin |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xueliang |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yanfeng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jianghua |e verfasserin |4 aut | |
| 700 | 1 | |a Du, Guocheng |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Long |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of microbiology and biotechnology |d 1997 |g 30(2020), 5 vom: 28. Mai, Seite 762-769 |w (DE-627)NLM093752350 |x 1738-8872 |7 nnns |
| 773 | 1 | 8 | |g volume:30 |g year:2020 |g number:5 |g day:28 |g month:05 |g pages:762-769 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.4014/jmb.1912.12008 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 30 |j 2020 |e 5 |b 28 |c 05 |h 762-769 | ||