Protective effects of melatonin on lung damage associated with one-lung ventilation : An experimental study
Copyright © 2020, Turkish Society of Cardiovascular Surgery..
BACKGROUND: This study aims to investigate the protective effect of melatonin on lung damage induced by one-lung ventilation in a rat model.
METHODS: A total of 20 healthy, Sprague-Dawley male rats were randomized into two equal groups as control (n=10) and melatonin groups (n=10). The control group underwent 60 min of one-lung ventilation, followed by 30 min of two-lung ventilation. In the melatonin group, the rats were administered 10 mg/kg melatonin intraperitoneally 10 min before the start of the experiment. At the end of both ventilation periods, tissue samples were obtained from the lungs of the control and melatonin groups for biochemical analysis and histopathological examinations. Tissue superoxide dismutase, malondialdehyde, and tumor necrosis factor-alpha levels were measured. Lung tissue samples were examined based on the presence and amount of alveolar congestion, intra-alveolar bleeding, and leukocyte and lymphocyte infiltration.
RESULTS: At the end of the study, lung tissue malondialdehyde (3.8±0.9 vs. 1.8±0.8 μM; p<0.001) and tumor necrosis factor-alpha levels (47.2±15.0 vs. 21.8±7.2 pg/mL; p<0.001) of the melatonin group were found to significantly decrease, compared to the control group. Superoxide dismutase levels of the melatonin group increased at the end of both ventilation periods, and the increase at the end of one-lung ventilation was found to be statistically significant (0.6±0.2 vs. 1.3±0.7 U/mL; p<0.05). Histopathological examination demonstrated that the tissue damage was less in the melatonin group. There was a significant decrease in the alveolar congestion in this group (p=0.0401). Although other histopathological parameters decreased in the melatonin group, no significant difference was found.
CONCLUSION: Our study results demonstrate that melatonin has protective effects on the lung damage induced by one-lung ventilation both at biochemical and histopathological levels in rats.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
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| Enthalten in: |
Turk gogus kalp damar cerrahisi dergisi - 28(2020), 1 vom: 20. Jan., Seite 151-157 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Durceylan, Erhan [VerfasserIn] |
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| Links: |
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| Themen: |
Histopathology |
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| Anmerkungen: |
Date Revised 13.04.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.5606/tgkdc.dergisi.2020.18261 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM307631028 |
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| 100 | 1 | |a Durceylan, Erhan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Protective effects of melatonin on lung damage associated with one-lung ventilation |b An experimental study |
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| 500 | |a Date Revised 13.04.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Copyright © 2020, Turkish Society of Cardiovascular Surgery. | ||
| 520 | |a BACKGROUND: This study aims to investigate the protective effect of melatonin on lung damage induced by one-lung ventilation in a rat model | ||
| 520 | |a METHODS: A total of 20 healthy, Sprague-Dawley male rats were randomized into two equal groups as control (n=10) and melatonin groups (n=10). The control group underwent 60 min of one-lung ventilation, followed by 30 min of two-lung ventilation. In the melatonin group, the rats were administered 10 mg/kg melatonin intraperitoneally 10 min before the start of the experiment. At the end of both ventilation periods, tissue samples were obtained from the lungs of the control and melatonin groups for biochemical analysis and histopathological examinations. Tissue superoxide dismutase, malondialdehyde, and tumor necrosis factor-alpha levels were measured. Lung tissue samples were examined based on the presence and amount of alveolar congestion, intra-alveolar bleeding, and leukocyte and lymphocyte infiltration | ||
| 520 | |a RESULTS: At the end of the study, lung tissue malondialdehyde (3.8±0.9 vs. 1.8±0.8 μM; p<0.001) and tumor necrosis factor-alpha levels (47.2±15.0 vs. 21.8±7.2 pg/mL; p<0.001) of the melatonin group were found to significantly decrease, compared to the control group. Superoxide dismutase levels of the melatonin group increased at the end of both ventilation periods, and the increase at the end of one-lung ventilation was found to be statistically significant (0.6±0.2 vs. 1.3±0.7 U/mL; p<0.05). Histopathological examination demonstrated that the tissue damage was less in the melatonin group. There was a significant decrease in the alveolar congestion in this group (p=0.0401). Although other histopathological parameters decreased in the melatonin group, no significant difference was found | ||
| 520 | |a CONCLUSION: Our study results demonstrate that melatonin has protective effects on the lung damage induced by one-lung ventilation both at biochemical and histopathological levels in rats | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Histopathology | |
| 650 | 4 | |a malondialdehyde | |
| 650 | 4 | |a melatonin | |
| 650 | 4 | |a one-lung ventilation | |
| 650 | 4 | |a superoxide dismutase | |
| 650 | 4 | |a tumor necrosis factor-alpha | |
| 700 | 1 | |a Aksu, Ebubekir |e verfasserin |4 aut | |
| 700 | 1 | |a Boztepe, Hacer |e verfasserin |4 aut | |
| 700 | 1 | |a Çengelli, Çiğdem |e verfasserin |4 aut | |
| 700 | 1 | |a Çiftçi Yılmaz, Evrim |e verfasserin |4 aut | |
| 700 | 1 | |a Dündar Kasapoğlu, Emine |e verfasserin |4 aut | |
| 700 | 1 | |a Erol, Kevser |e verfasserin |4 aut | |
| 700 | 1 | |a Sivrikoz, Muammer Cumhur |e verfasserin |4 aut | |
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