Nanocapsulation of horseradish peroxidase (HRP) enhances enzymatic performance in removing phenolic compounds
Copyright © 2020 Elsevier B.V. All rights reserved..
As ubiquitous environmental pollutants, phenolic compounds are requested to be efficiently removed from wastewater. Enzymes, such as Horseradish peroxidase (HRP), have been demonstrated with great potential in removing phenolic compounds. Different from the general immobilization technology, the encapsulation of individual enzymes within nanogel has been employed in this work. Here we show that, the encapsulated HRP could remarkably enhance enzymatic performance, including thermostability, catalytic efficiency, environmental tolerance and, most importantly, the biodegradation of phenolic compounds. For instance, the removal efficiencies of phenol and BPA increased by 7-fold and 3.5-fold, respectively. On the other hand, the diverted removal efficiencies were obtained for a series of phenolic compounds. Based on molecular modelling, the biodegradabilities of phenolic compounds were rationalized according to their redox potentials and binding affinities with enzymes. In summary, our work indicates that the nanocapsulation of enzyme should be a promising strategy in removing different types of phenolic compounds from wastewater.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:150 |
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| Enthalten in: |
International journal of biological macromolecules - 150(2020) vom: 01. Mai, Seite 814-822 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Shan [VerfasserIn] |
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| Links: |
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| Themen: |
339NCG44TV |
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| Anmerkungen: |
Date Completed 04.01.2021 Date Revised 07.12.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijbiomac.2020.02.043 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM306305380 |
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| 245 | 1 | 0 | |a Nanocapsulation of horseradish peroxidase (HRP) enhances enzymatic performance in removing phenolic compounds |
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| 500 | |a Date Revised 07.12.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a As ubiquitous environmental pollutants, phenolic compounds are requested to be efficiently removed from wastewater. Enzymes, such as Horseradish peroxidase (HRP), have been demonstrated with great potential in removing phenolic compounds. Different from the general immobilization technology, the encapsulation of individual enzymes within nanogel has been employed in this work. Here we show that, the encapsulated HRP could remarkably enhance enzymatic performance, including thermostability, catalytic efficiency, environmental tolerance and, most importantly, the biodegradation of phenolic compounds. For instance, the removal efficiencies of phenol and BPA increased by 7-fold and 3.5-fold, respectively. On the other hand, the diverted removal efficiencies were obtained for a series of phenolic compounds. Based on molecular modelling, the biodegradabilities of phenolic compounds were rationalized according to their redox potentials and binding affinities with enzymes. In summary, our work indicates that the nanocapsulation of enzyme should be a promising strategy in removing different types of phenolic compounds from wastewater | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Enzyme encapsulation | |
| 650 | 4 | |a Horseradish peroxidase | |
| 650 | 4 | |a Phenolic compounds | |
| 650 | 7 | |a Enzymes, Immobilized |2 NLM | |
| 650 | 7 | |a Nanocapsules |2 NLM | |
| 650 | 7 | |a Phenols |2 NLM | |
| 650 | 7 | |a Waste Water |2 NLM | |
| 650 | 7 | |a Phenol |2 NLM | |
| 650 | 7 | |a 339NCG44TV |2 NLM | |
| 650 | 7 | |a Horseradish Peroxidase |2 NLM | |
| 650 | 7 | |a EC 1.11.1.- |2 NLM | |
| 700 | 1 | |a Huang, Biyan |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Guiqin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Juying |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yantao |c Prof |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Li |e verfasserin |4 aut | |
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