Pregnane glycoside from Huernia saudi-arabica as latent schistosomicidal mediator
Development of a novel agent for control of schistosomiasis is a mandate. In-vitro anti-schistosomal activity of the aerial parts of Huernia saudi-arabica were examined. Chromatographic investigations of the ethanol extract (EE) were afforded three compounds. Pregnane glycoside (CI) 12-β-p-hydroxy-benzoyl-20-O-acetyl-boucerin-3-O-β-D-glucopyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside, in addition to two flavonoids (CII) luteolin-4'-O-β-D-neohesperidoside and (CIII)quercetin-3-rutinoside were recognized via spectral analysis. The schistosomicidal effects were evaluated using scanning electron microscope (SEM). In-vitro bioassays on the viability (mobility, morphological changes and mortality) of Schistosoma mansoni adults, cercariae, miracidia and eggs at different concentrations 2.5, 5, 12.5, 25 and 50 μg/ml of EE and 2.6, 5.2, 13, 26 and 52 μM of CI in incubation times 1,2,4,6,12hrs were carried out. EE and CI evidenced in-vitro anti-schistosomal activity with a dose and incubation time-dependent fashion. The effect of EE and CI was evident by the topography damage showed by SEM. EE proved moderate in-vitro cytotoxicity with IC50 of 8.48 µg/ml.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2020 |
---|---|
Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
---|---|
Enthalten in: |
Natural product research - 34(2020), 2 vom: 01. Jan., Seite 311-316 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
El Sayed, Abeer M [VerfasserIn] |
---|
Links: |
---|
Themen: |
Cytotoxicity |
---|
Anmerkungen: |
Date Completed 10.03.2020 Date Revised 10.03.2020 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1080/14786419.2018.1528584 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM290373743 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM290373743 | ||
003 | DE-627 | ||
005 | 20231225064606.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2020 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1080/14786419.2018.1528584 |2 doi | |
028 | 5 | 2 | |a pubmed24n0967.xml |
035 | |a (DE-627)NLM290373743 | ||
035 | |a (NLM)30404547 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a El Sayed, Abeer M |e verfasserin |4 aut | |
245 | 1 | 0 | |a Pregnane glycoside from Huernia saudi-arabica as latent schistosomicidal mediator |
264 | 1 | |c 2020 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 10.03.2020 | ||
500 | |a Date Revised 10.03.2020 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Development of a novel agent for control of schistosomiasis is a mandate. In-vitro anti-schistosomal activity of the aerial parts of Huernia saudi-arabica were examined. Chromatographic investigations of the ethanol extract (EE) were afforded three compounds. Pregnane glycoside (CI) 12-β-p-hydroxy-benzoyl-20-O-acetyl-boucerin-3-O-β-D-glucopyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside, in addition to two flavonoids (CII) luteolin-4'-O-β-D-neohesperidoside and (CIII)quercetin-3-rutinoside were recognized via spectral analysis. The schistosomicidal effects were evaluated using scanning electron microscope (SEM). In-vitro bioassays on the viability (mobility, morphological changes and mortality) of Schistosoma mansoni adults, cercariae, miracidia and eggs at different concentrations 2.5, 5, 12.5, 25 and 50 μg/ml of EE and 2.6, 5.2, 13, 26 and 52 μM of CI in incubation times 1,2,4,6,12hrs were carried out. EE and CI evidenced in-vitro anti-schistosomal activity with a dose and incubation time-dependent fashion. The effect of EE and CI was evident by the topography damage showed by SEM. EE proved moderate in-vitro cytotoxicity with IC50 of 8.48 µg/ml | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a SEM | |
650 | 4 | |a Schistosoma mansoni; Huernia saudi-arabica | |
650 | 4 | |a cytotoxicity | |
650 | 4 | |a pregnane glycoside | |
650 | 7 | |a Flavonoids |2 NLM | |
650 | 7 | |a Glycosides |2 NLM | |
650 | 7 | |a Plant Extracts |2 NLM | |
650 | 7 | |a Pregnanes |2 NLM | |
650 | 7 | |a Schistosomicides |2 NLM | |
650 | 7 | |a pregnane glycoside |2 NLM | |
700 | 1 | |a Basyoni, Maha M |e verfasserin |4 aut | |
700 | 1 | |a ElGayed, Sabah H |e verfasserin |4 aut | |
700 | 1 | |a El-Badry, Ayman A |e verfasserin |4 aut | |
700 | 1 | |a Abdel-Sattar, Essam |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Natural product research |d 2003 |g 34(2020), 2 vom: 01. Jan., Seite 311-316 |w (DE-627)NLM124466303 |x 1478-6427 |7 nnns |
773 | 1 | 8 | |g volume:34 |g year:2020 |g number:2 |g day:01 |g month:01 |g pages:311-316 |
856 | 4 | 0 | |u http://dx.doi.org/10.1080/14786419.2018.1528584 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 34 |j 2020 |e 2 |b 01 |c 01 |h 311-316 |