Differential modulation of adenylyl cyclases I and II by various G beta subunits
The accepted dogma concerning the regulation of adenylyl cyclase (AC) activity by G beta gamma dimers states that the various isoforms of AC respond differently to the presence of free G beta gamma. It has been demonstrated that AC I activity is inhibited and AC II activity is stimulated by G beta gamma subunits. This result does not address the possible differences in modulation that may exist among the different G beta gamma heterodimers. Six isoforms of G beta and 12 isoforms of G gamma have been cloned to date. We have established a cell transfection system in which G beta and G gamma cDNAs were cotransfected with either AC isoform I or II and the activity of these isoforms was determined. We found that while AC I activity was inhibited by both G beta 1/gamma 2 and G beta 5/gamma 2 combinations, AC II responded differentially and was stimulated by G beta 1/gamma 2 and inhibited by G beta 5/gamma 2. This finding demonstrates differential modulatory activity by different combinations of G beta gamma on the same AC isoform and demonstrates another level of complexity within the AC signaling system.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
1998 |
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| Erschienen: |
1998 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:273 |
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| Enthalten in: |
The Journal of biological chemistry - 273(1998), 4 vom: 23. Jan., Seite 2273-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bayewitch, M L [VerfasserIn] |
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| Themen: |
Adenylyl Cyclases |
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| Anmerkungen: |
Date Completed 03.03.1998 Date Revised 09.02.2021 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM09385238X |
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| 100 | 1 | |a Bayewitch, M L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Differential modulation of adenylyl cyclases I and II by various G beta subunits |
| 264 | 1 | |c 1998 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 03.03.1998 | ||
| 500 | |a Date Revised 09.02.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The accepted dogma concerning the regulation of adenylyl cyclase (AC) activity by G beta gamma dimers states that the various isoforms of AC respond differently to the presence of free G beta gamma. It has been demonstrated that AC I activity is inhibited and AC II activity is stimulated by G beta gamma subunits. This result does not address the possible differences in modulation that may exist among the different G beta gamma heterodimers. Six isoforms of G beta and 12 isoforms of G gamma have been cloned to date. We have established a cell transfection system in which G beta and G gamma cDNAs were cotransfected with either AC isoform I or II and the activity of these isoforms was determined. We found that while AC I activity was inhibited by both G beta 1/gamma 2 and G beta 5/gamma 2 combinations, AC II responded differentially and was stimulated by G beta 1/gamma 2 and inhibited by G beta 5/gamma 2. This finding demonstrates differential modulatory activity by different combinations of G beta gamma on the same AC isoform and demonstrates another level of complexity within the AC signaling system | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, P.H.S. | |
| 650 | 7 | |a GTP-Binding Proteins |2 NLM | |
| 650 | 7 | |a EC 3.6.1.- |2 NLM | |
| 650 | 7 | |a Adenylyl Cyclases |2 NLM | |
| 650 | 7 | |a EC 4.6.1.1 |2 NLM | |
| 650 | 7 | |a adenylyl cyclase 1 |2 NLM | |
| 650 | 7 | |a EC 4.6.1.1 |2 NLM | |
| 650 | 7 | |a adenylyl cyclase 2 |2 NLM | |
| 650 | 7 | |a EC 4.6.1.1 |2 NLM | |
| 700 | 1 | |a Avidor-Reiss, T |e verfasserin |4 aut | |
| 700 | 1 | |a Levy, R |e verfasserin |4 aut | |
| 700 | 1 | |a Pfeuffer, T |e verfasserin |4 aut | |
| 700 | 1 | |a Nevo, I |e verfasserin |4 aut | |
| 700 | 1 | |a Simonds, W F |e verfasserin |4 aut | |
| 700 | 1 | |a Vogel, Z |e verfasserin |4 aut | |
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