Rottlerin-Liposome Inhibits Endocytosis of Feline Infectious Peritonitis Virus Infection Through PKCδ Dependent Manner
Rottlerin(R) is a natural extract from Mallotus philippensis with antiviral properties. Feline Infectious Peritonitis (FIP) is a fatal disease characterized by systemic granulomatous inflammation and high mortality, with no established prevention or cure. We investigated antiviral effect of liposome loaded R, Rottlerin-liposome(RL) against Feline infectious peritonitis virus (FIPV), the causative agent. We demonstrated that R and RL inhibited FIPV replication in dose dependently with a PKCδ related manner, not only in the early endocytosis step but also in the late step of replication. RL resolved the low solubility issue of rottlerin and improved its inhibition efficacy at the cellular level. Based on these findings, we suggest that RL has a value for further research as a potential antiviral agent against FIPV..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
Preprints.org - (2023) vom: 05. Juni Zur Gesamtaufnahme - year:2023 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Choi, Jong-Chul [VerfasserIn] |
|---|
| Links: |
|---|
| doi: |
10.20944/preprints202304.0106.v1 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
preprintsorg039207005 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | preprintsorg039207005 | ||
| 003 | DE-627 | ||
| 005 | 20231205145401.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230410s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.20944/preprints202304.0106.v1 |2 doi | |
| 024 | 7 | |a 10.20944/preprints202304.0106.v1 |2 doi | |
| 035 | |a (DE-627)preprintsorg039207005 | ||
| 035 | |a (preprintsorg)10.20944/preprints202304.0106 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Choi, Jong-Chul |e verfasserin |0 (orcid)0000-0003-3099-6040 |4 aut | |
| 245 | 1 | 0 | |a Rottlerin-Liposome Inhibits Endocytosis of Feline Infectious Peritonitis Virus Infection Through PKCδ Dependent Manner |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Rottlerin(R) is a natural extract from Mallotus philippensis with antiviral properties. Feline Infectious Peritonitis (FIP) is a fatal disease characterized by systemic granulomatous inflammation and high mortality, with no established prevention or cure. We investigated antiviral effect of liposome loaded R, Rottlerin-liposome(RL) against Feline infectious peritonitis virus (FIPV), the causative agent. We demonstrated that R and RL inhibited FIPV replication in dose dependently with a PKCδ related manner, not only in the early endocytosis step but also in the late step of replication. RL resolved the low solubility issue of rottlerin and improved its inhibition efficacy at the cellular level. Based on these findings, we suggest that RL has a value for further research as a potential antiviral agent against FIPV. | ||
| 700 | 1 | |a Jung, Sung-Won |4 aut | |
| 700 | 1 | |a Choi, In-Yeong |4 aut | |
| 700 | 1 | |a Kang, Yeong-Lim |4 aut | |
| 700 | 1 | |a Lee, Dong-Hun |4 aut | |
| 700 | 1 | |a Lee, Sang-Won |4 aut | |
| 700 | 1 | |a Park, Seung-Yong |4 aut | |
| 700 | 1 | |a Song, Chang-Seon |0 (orcid)0000-0002-4158-6402 |4 aut | |
| 700 | 1 | |a Choi, In-Soo |4 aut | |
| 700 | 1 | |a Lee, Joong-Bok |0 (orcid)0000-0003-1074-8162 |4 aut | |
| 700 | 1 | |a Oh, Changin |0 (orcid)0000-0003-2300-6360 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Preprints.org |g (2023) vom: 05. Juni |
| 773 | 1 | 8 | |g year:2023 |g day:05 |g month:06 |
| 856 | 4 | 0 | |u https://doi.org/10.3390/vetsci10060380 |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://dx.doi.org/10.20944/preprints202304.0106.v1 |z kostenfrei |3 Volltext |
| 912 | |a preprintsorg | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 05 |c 06 | ||