MiR-145 modulation of TGF-β pathway attenuates EMT inhibition of Wilms’Tumor cell proliferation, invasion and migration
Abstract To explore the impact of miR-145 on the proliferation, migration, and invasion of Wilms' tumor G401 cells by attenuating EMT through modulation of the TGF-β pathway.A Wilms' tumor G401 cell line overexpressing miR-145 and a blank control group were established.The proliferation of Wilms' tumor cells was assessed using the CCK8 assay and colony formation experiments.The influence of miR-145 overexpression on the migration and invasion capabilities of these cells was evaluated through Transwell assays.Western blotting was used to analyze the expression of TGF-β pathway-related proteins,EMT-related proteins,and apoptosis-related proteins.Additionally,changes in apoptotic proteins post-treatment with cisplatin were examined via Western blot.An orthotopic Wilms' tumor mouse model was developed to assess the effect of miR-145 overexpression on tumor growth and cisplatin treatment response.CCK8,clonogenic formation,and Transwell assays indicated that miR-145 overexpression group exhibited reduced proliferation,migration,and invasion of Wilms' tumor cells compared to the control.Western blot results demonstrated that miR-145 overexpression suppressed EMT in Wilms' tumor by inhibiting the Smad-mediated TGF-β pathway.MiR-145 significantly induced apoptosis in these cells and improved the therapeutic response to cisplatin,diminishing drug resistance.The orthotopic Wilms' tumor mouse model validated that miR-145 inhibited primary tumor growth in the Wilms' tumor xenograft and enhanced cisplatin treatment response.Taken together,miR-145 inhibits EMT in Wilms' tumor by suppressing the Smad-mediated TGF-β pathway,effectively reducing cell proliferation,migration,and invasion.The miR-145/Smad/TGF-β/EMT axis emerges as a potential therapeutic target for Wilms' tumor..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
ResearchSquare.com - (2024) vom: 20. März Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhou, Jun [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.21203/rs.3.rs-3991565/v1 |
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| PPN (Katalog-ID): |
XRA042992583 |
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| 520 | |a Abstract To explore the impact of miR-145 on the proliferation, migration, and invasion of Wilms' tumor G401 cells by attenuating EMT through modulation of the TGF-β pathway.A Wilms' tumor G401 cell line overexpressing miR-145 and a blank control group were established.The proliferation of Wilms' tumor cells was assessed using the CCK8 assay and colony formation experiments.The influence of miR-145 overexpression on the migration and invasion capabilities of these cells was evaluated through Transwell assays.Western blotting was used to analyze the expression of TGF-β pathway-related proteins,EMT-related proteins,and apoptosis-related proteins.Additionally,changes in apoptotic proteins post-treatment with cisplatin were examined via Western blot.An orthotopic Wilms' tumor mouse model was developed to assess the effect of miR-145 overexpression on tumor growth and cisplatin treatment response.CCK8,clonogenic formation,and Transwell assays indicated that miR-145 overexpression group exhibited reduced proliferation,migration,and invasion of Wilms' tumor cells compared to the control.Western blot results demonstrated that miR-145 overexpression suppressed EMT in Wilms' tumor by inhibiting the Smad-mediated TGF-β pathway.MiR-145 significantly induced apoptosis in these cells and improved the therapeutic response to cisplatin,diminishing drug resistance.The orthotopic Wilms' tumor mouse model validated that miR-145 inhibited primary tumor growth in the Wilms' tumor xenograft and enhanced cisplatin treatment response.Taken together,miR-145 inhibits EMT in Wilms' tumor by suppressing the Smad-mediated TGF-β pathway,effectively reducing cell proliferation,migration,and invasion.The miR-145/Smad/TGF-β/EMT axis emerges as a potential therapeutic target for Wilms' tumor. | ||
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| 700 | 1 | |a Zhu, Jian |4 aut | |
| 700 | 1 | |a Song, Jialun |4 aut | |
| 700 | 1 | |a Wang, Peng |4 aut | |
| 700 | 1 | |a Zhao, Xinxin |4 aut | |
| 700 | 1 | |a Liu, Ziping |4 aut | |
| 700 | 1 | |a Lu, Songxian |4 aut | |
| 700 | 1 | |a Shi, Hongguang |4 aut | |
| 700 | 1 | |a Feng, Xiaojuan |4 aut | |
| 700 | 1 | |a Zheng, Mingjun |4 aut | |
| 700 | 1 | |a Du, Junpeng |4 aut | |
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