Commercial Real-Time PCR Kit Results for the Detection of Helicobacter pylori and Clarithromycin Resistance in Stool Samples

Abstract Helicobacter pylori (H. pylori) is a bacterium that infects half of the world's population and unless treated, colonization usually persists lifelong. Non-invasive tests for the detection of H. pylori infections have indeed been valuable for their ease of use and reduced discomfort for patients. However, many non-invasive tests primarily focus on identifying the presence of bacteria rather than determining the antimicrobial susceptibility. The aims of this study were to detect H. pylori infections and the point mutations that led to clarithromycin resistance by using RT-PCR from gastric biopsy and stool samples; to perform antibiotic susceptibility test and to compare the results of antibiotic susuceptibility tests to the detected point mutations that led to clarithromycin resistance. A total of 73 patients attending to Gastroenterology Department of Hacettepe University Hospital between January 2021 and July 2022 were included in the study. For various reasons 10 of the patients were excluded from the study. Thus, consent forms were obtained from all 63 patients. Stool samples were collected from each participant within 1–7 days following gastroduodenal endoscopy. Subsequently, the samples were homogenized and stored at -20°C for further examination using RT-PCR. Gastric biopsy culture, rapid urease test, histopathological examination, HpSA and gastric biopsy RT-PCR methods were performed for the diagnosis of H. pylori. In the study, a combination of two positive methods were accepted as the reference standard. Among the 63 patients included in the study, 42.9% tested H. pylori positive, while 57.1% tested H. pylori negative. When the reference results were compared to stool RT-PCR; for H. pylori detection, the sensitivity was 66.6%, specificity was 100%, positive predictive value was 100%, negative predictive value was 80.0%, and the diagnostic accuracy was 85.7%. In the detection of clarithromycin point mutations from stool samples with RT-PCR, the overall agreement was 65% when compared to the results obtained from gastric biopsy RT-PCR. In conclusion, the outcomes of this study indicate that utilizing RT-PCR from stool samples holds potential as a diagnostic tool for detecting H. pylori. This is especially relevant in circumstances where invasive tests present challenges, such as in pediatric patients, pregnant women, and during the follow-up of treatment..

Medienart:	Preprint

Erscheinungsjahr:	2024
Erschienen:	2024

Enthalten in:	ResearchSquare.com - (2024) vom: 21. Feb. Zur Gesamtaufnahme - year:2024

Sprache:	Englisch

Beteiligte Personen:	İnal, Neşe [VerfasserIn] Şahin, Tevhide [VerfasserIn] Balaban, H. Yasemin [VerfasserIn] Şimşek, Cem [VerfasserIn] Şimşek, Halis [VerfasserIn] Akyön, Yakut [VerfasserIn]

Links:	Volltext [kostenfrei]

Themen:	570 Biology

doi:	10.21203/rs.3.rs-3959435/v1

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	XRA042592046