Gut microbiota from patients with COVID-19 cause alterations in mice that resemble post-COVID symptoms
Abstract Long-term sequelae after Coronavirus disease (COVID)-19 are frequent and of major concern. SARS-CoV-2 infection affects the host's gut microbiota, which is linked with disease severity in patients with COVID-19. We report here that the gut microbiota of post-COVID subjects had a remarkable predominance of Enterobacteriaceae strains with antibiotic-resistance phenotype compared to healthy controls. Additionally, short-chain fatty acids (SCFA) levels were reduced in their feces. Fecal transplant from post-COVID subjects to germ-free mice led to lung inflammation and worst outcomes during pulmonary infection by multidrug-resistant Klebsiella pneumoniae. Transplanted mice also had poorer cognitive performance. Overall, we show prolonged impacts of SARS-CoV-2 infection in the gut microbiota that persist after subjects have cleared the virus. Together, these data demonstrate that the gut microbiota can directly contribute to post-COVID sequelae, suggesting that it may be a potential therapeutic target..
Medienart: |
Preprint |
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Erscheinungsjahr: |
2023 |
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Erschienen: |
2023 |
Enthalten in: |
ResearchSquare.com - (2023) vom: 12. Apr. Zur Gesamtaufnahme - year:2023 |
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Sprache: |
Englisch |
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Links: |
Volltext [kostenfrei] |
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Themen: |
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doi: |
10.21203/rs.3.rs-1756189/v2 |
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funding: |
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PPN (Katalog-ID): |
XRA039243567 |
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520 | |a Abstract Long-term sequelae after Coronavirus disease (COVID)-19 are frequent and of major concern. SARS-CoV-2 infection affects the host's gut microbiota, which is linked with disease severity in patients with COVID-19. We report here that the gut microbiota of post-COVID subjects had a remarkable predominance of Enterobacteriaceae strains with antibiotic-resistance phenotype compared to healthy controls. Additionally, short-chain fatty acids (SCFA) levels were reduced in their feces. Fecal transplant from post-COVID subjects to germ-free mice led to lung inflammation and worst outcomes during pulmonary infection by multidrug-resistant Klebsiella pneumoniae. Transplanted mice also had poorer cognitive performance. Overall, we show prolonged impacts of SARS-CoV-2 infection in the gut microbiota that persist after subjects have cleared the virus. Together, these data demonstrate that the gut microbiota can directly contribute to post-COVID sequelae, suggesting that it may be a potential therapeutic target. | ||
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700 | 1 | |a Engel, Daiane F |4 aut | |
700 | 1 | |a Ricci, Mayra Fernanda |4 aut | |
700 | 1 | |a Cruz, Clênio Silva |4 aut | |
700 | 1 | |a Lopes, Icaro Santos |4 aut | |
700 | 1 | |a Alves, Daniele Almeida |4 aut | |
700 | 1 | |a Auriol, Mirna d’ |4 aut | |
700 | 1 | |a Magalhães, João |4 aut | |
700 | 1 | |a Zuccoli, Giuliana S. |4 aut | |
700 | 1 | |a Smith, Bradley Joseph |4 aut | |
700 | 1 | |a Carregari, Victor Corasolla |4 aut | |
700 | 1 | |a Machado, Elayne Cristina |4 aut | |
700 | 1 | |a Rocha, Victor M. |4 aut | |
700 | 1 | |a Carvalho, Toniana G. |4 aut | |
700 | 1 | |a Lacerda, Larisse de Souza Barbosa |4 aut | |
700 | 1 | |a Pimenta, Jordane C. |4 aut | |
700 | 1 | |a Galvão, Izabela |4 aut | |
700 | 1 | |a Silva, Mariana Aganetti |4 aut | |
700 | 1 | |a Rosa, Erika da Silva |4 aut | |
700 | 1 | |a Cassali, Geovanni Dantas |4 aut | |
700 | 1 | |a Garcia, Cristiana C. |4 aut | |
700 | 1 | |a Teixeira, Mauro Martins |4 aut | |
700 | 1 | |a Coelho, Leiliane |4 aut | |
700 | 1 | |a Ribeiro, Fabiola Mara |4 aut | |
700 | 1 | |a Martins, Flaviano S. |4 aut | |
700 | 1 | |a Saia, Rafael Simone |4 aut | |
700 | 1 | |a Costa, Vivian Vasconcelos |4 aut | |
700 | 1 | |a Martins-de-Souza, Daniel |4 aut | |
700 | 1 | |a Marques, João T. |4 aut | |
700 | 1 | |a Aguiar, Eric R. G. R. |4 aut | |
700 | 1 | |a Vieira, Angelica T. |4 aut | |
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