Comparison of infection and human immune responses of two SARS-CoV-2 strains in a humanized AdV-hACE2 NIKO mouse model
Abstract The COVID-19 pandemic has sickened millions, cost lives and has devastated the global economy. Various animal models for experimental infection with SARS-CoV-2 have played a key role in many aspects of COVID-19 research. Here, we describe a humanized AdV-hACE2 NOD-SCID IL2Rγ-/- (NIKO) mouse model and compared infection with ancestral and mutant (SARS-CoV-2-∆382) strains of SARS-CoV-2. Immune cell infiltration, inflammation, lung damage and pro-inflammatory cytokines and chemokines was observed in humanized AdV-hACE2 NIKO mice. Humanized AdV-hACE2 NIKO mice infected with the WT and mutant SARS-CoV-2 strain had lung inflammation and production of pro-inflammatory cytokines and chemokines. This model can aid in examining the pathological basis of SARS-CoV-2 infection in a human immune environment and evaluation of therapeutic interventions..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
ResearchSquare.com - (2023) vom: 17. Okt. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yong, Kylie Su Mei [VerfasserIn] |
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| doi: |
10.21203/rs.3.rs-2672493/v1 |
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| 245 | 1 | 0 | |a Comparison of infection and human immune responses of two SARS-CoV-2 strains in a humanized AdV-hACE2 NIKO mouse model |
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| 520 | |a Abstract The COVID-19 pandemic has sickened millions, cost lives and has devastated the global economy. Various animal models for experimental infection with SARS-CoV-2 have played a key role in many aspects of COVID-19 research. Here, we describe a humanized AdV-hACE2 NOD-SCID IL2Rγ-/- (NIKO) mouse model and compared infection with ancestral and mutant (SARS-CoV-2-∆382) strains of SARS-CoV-2. Immune cell infiltration, inflammation, lung damage and pro-inflammatory cytokines and chemokines was observed in humanized AdV-hACE2 NIKO mice. Humanized AdV-hACE2 NIKO mice infected with the WT and mutant SARS-CoV-2 strain had lung inflammation and production of pro-inflammatory cytokines and chemokines. This model can aid in examining the pathological basis of SARS-CoV-2 infection in a human immune environment and evaluation of therapeutic interventions. | ||
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