Forensic analysis of novel SARS2r-CoV identified in game animal datasets in China shows evolutionary relationship to Pangolin GX CoV clade and apparent genetic experimentation
Abstract Pangolins are the only animals other than bats proposed to have been infected with SARS-CoV-2 related coronaviruses (SARS2r-CoVs) prior to the COVID-19 pandemic. Here we examine the novel SARS2r-CoV we previously identified in game animal metatranscriptomic datasets sequenced by He et al. (2022) and find that sections of the partial genome phylogenetically group with Guangxi (GX) pangolin CoVs (GX PCoVs) while the full RdRp sequence groups with bat-SL-CoVZC45. While the novel SARS2r-CoV is found in 6 pangolin datasets, the same CoV is also found in 10 additional NGS datasets from 5 separate mammalian species and is likely related to contamination by a laboratory researched virus. We find that NGS datasets containing the novel SARS2r-CoV are contaminated with significant Homo sapiens genetic material, and numerous viruses not associated with the host animals sampled. We further identify the dominant human haplogroup of the contaminating H.sapiens genetic material to be F1c1a1, which is of East Asian provenance. The association of this novel CoV with both bat CoV and the Guangxi pangolin CoV (GX PCoV) clades is an important step towards identifying the origin of the GX PCoVs..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
ResearchSquare.com - (2022) vom: 03. Aug. Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jones, Adrian [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.21203/rs.3.rs-1836803/v1 |
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| 245 | 1 | 0 | |a Forensic analysis of novel SARS2r-CoV identified in game animal datasets in China shows evolutionary relationship to Pangolin GX CoV clade and apparent genetic experimentation |
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| 520 | |a Abstract Pangolins are the only animals other than bats proposed to have been infected with SARS-CoV-2 related coronaviruses (SARS2r-CoVs) prior to the COVID-19 pandemic. Here we examine the novel SARS2r-CoV we previously identified in game animal metatranscriptomic datasets sequenced by He et al. (2022) and find that sections of the partial genome phylogenetically group with Guangxi (GX) pangolin CoVs (GX PCoVs) while the full RdRp sequence groups with bat-SL-CoVZC45. While the novel SARS2r-CoV is found in 6 pangolin datasets, the same CoV is also found in 10 additional NGS datasets from 5 separate mammalian species and is likely related to contamination by a laboratory researched virus. We find that NGS datasets containing the novel SARS2r-CoV are contaminated with significant Homo sapiens genetic material, and numerous viruses not associated with the host animals sampled. We further identify the dominant human haplogroup of the contaminating H.sapiens genetic material to be F1c1a1, which is of East Asian provenance. The association of this novel CoV with both bat CoV and the Guangxi pangolin CoV (GX PCoV) clades is an important step towards identifying the origin of the GX PCoVs. | ||
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