Interaction between TGF-β1 and long non-coding RNA 4.9 in a model of latent infection with human cytomegalovirus
Abstract To investigate the interaction between long non-coding RNA4.9 (lncRNA4.9) and transforming growth factor-β1 (TGF-β1) in the model of HCMV DNA. The experiment was divided into 5 groups: NC group, PCDNA-lncRNA4.9 group, SH-lncRNA4.9 group, LV-TGF-β1) Group, and SH-lncRNA4.9 group. Compared with the NC group, overexpression of lncRNA4.9 could significantly increase (P <0.05) the expression of TGF-β1 and decrease with time. The relative expression of HCMV DNA was significantly decreased (P <0.05) with the overexpression of lncRNA4.9. Compared with the NC group, overexpression of TGF-β1 significantly increased the expression of lncRNA4.9 and decreased with time. The relative expression of HCMV DNA was significantly decreased (P <0.05) with the overexpression of TGF-β1. However, silencing TGF-β1 could not reduce the relative expression of lncRNA4.9, but increased the relative expression of lncRNA4.9 on day 5, and the difference was statistically significant (P <0.05). Silencing lncRNA4.9 could not reduce expression of TGF-β1, but increased expression of lncRNA4.9 on day 5 (P <0.05). Overexpression of TGF-β1 could increase the relative expression level of lncRNA4.9 and decrease with time, and reduce the relative expression level of HCMV DNA, thus promoting HCMV into the latent infection state..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
ResearchSquare.com - (2022) vom: 29. Juni Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yi, Xiaolian [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.21203/rs.3.rs-1767246/v1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
XRA036397547 |
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| 520 | |a Abstract To investigate the interaction between long non-coding RNA4.9 (lncRNA4.9) and transforming growth factor-β1 (TGF-β1) in the model of HCMV DNA. The experiment was divided into 5 groups: NC group, PCDNA-lncRNA4.9 group, SH-lncRNA4.9 group, LV-TGF-β1) Group, and SH-lncRNA4.9 group. Compared with the NC group, overexpression of lncRNA4.9 could significantly increase (P <0.05) the expression of TGF-β1 and decrease with time. The relative expression of HCMV DNA was significantly decreased (P <0.05) with the overexpression of lncRNA4.9. Compared with the NC group, overexpression of TGF-β1 significantly increased the expression of lncRNA4.9 and decreased with time. The relative expression of HCMV DNA was significantly decreased (P <0.05) with the overexpression of TGF-β1. However, silencing TGF-β1 could not reduce the relative expression of lncRNA4.9, but increased the relative expression of lncRNA4.9 on day 5, and the difference was statistically significant (P <0.05). Silencing lncRNA4.9 could not reduce expression of TGF-β1, but increased expression of lncRNA4.9 on day 5 (P <0.05). Overexpression of TGF-β1 could increase the relative expression level of lncRNA4.9 and decrease with time, and reduce the relative expression level of HCMV DNA, thus promoting HCMV into the latent infection state. | ||
| 700 | 1 | |a Liu, Lifang |e verfasserin |4 aut | |
| 700 | 1 | |a Tao, Ran |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Huamei |e verfasserin |4 aut | |
| 700 | 1 | |a He, lin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yujie |e verfasserin |4 aut | |
| 700 | 1 | |a Shang, Shiqiang |e verfasserin |4 aut | |
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