Metagenomic sequencing analysis of bacterial biodiversity in the treatment of surplus sludge and chicken manure mixtures by black soldier fly larvae (Hermetia illucens)
Abstract The disposal of combined organic waste by black soldier fly larvae (Hermetia illucens) has drawn broad attention. However, changes of microflora during the treatment are lacking. In this study, livestock manure and surplus sludge were chosen as the target organic waste due to their wide produced in breeding industry and water treatment industry. Black solider fly (BSF) larvae were used to treat the mixture of chicken manure and surplus sludge for a week. Bacterial biodiversity of BSF larvae production residues were investigated with metagenomic sequencing technology to identify specific flora. The change trend of BSF larvae production residue derived from the mixture of chicken manure and surplus were closer to that resulting from chicken manure. Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes are the main bacteria in phylum level with the content of 75.39%,14.63%,5.69% and 2.42% in abundance. Bacilli were the major bacteria with 71.61% in class level. Pseudogracilibacillus(11.08%), Virgibacillus(9.86%) and Oceanobacillus(6.39%) were the key component in Bacilli. Erysipelotrichales as the probiotics of insect, also as pathogenic bacteria to poultry and human were found massive proliferation with the abundance of 2.71%. Overall, BSF has proved to be a sustainable management for organic waste by reducing its amount and whether the BSF larvae production residue can be used as organic fertilizer directly was controversial result from the bacterial biodiversity analysis..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
ResearchSquare.com - (2022) vom: 02. Mai Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Lei [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.21203/rs.3.rs-1564172/v1 |
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| PPN (Katalog-ID): |
XRA035817720 |
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| 245 | 1 | 0 | |a Metagenomic sequencing analysis of bacterial biodiversity in the treatment of surplus sludge and chicken manure mixtures by black soldier fly larvae (Hermetia illucens) |
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| 520 | |a Abstract The disposal of combined organic waste by black soldier fly larvae (Hermetia illucens) has drawn broad attention. However, changes of microflora during the treatment are lacking. In this study, livestock manure and surplus sludge were chosen as the target organic waste due to their wide produced in breeding industry and water treatment industry. Black solider fly (BSF) larvae were used to treat the mixture of chicken manure and surplus sludge for a week. Bacterial biodiversity of BSF larvae production residues were investigated with metagenomic sequencing technology to identify specific flora. The change trend of BSF larvae production residue derived from the mixture of chicken manure and surplus were closer to that resulting from chicken manure. Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes are the main bacteria in phylum level with the content of 75.39%,14.63%,5.69% and 2.42% in abundance. Bacilli were the major bacteria with 71.61% in class level. Pseudogracilibacillus(11.08%), Virgibacillus(9.86%) and Oceanobacillus(6.39%) were the key component in Bacilli. Erysipelotrichales as the probiotics of insect, also as pathogenic bacteria to poultry and human were found massive proliferation with the abundance of 2.71%. Overall, BSF has proved to be a sustainable management for organic waste by reducing its amount and whether the BSF larvae production residue can be used as organic fertilizer directly was controversial result from the bacterial biodiversity analysis. | ||
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| 700 | 1 | |a He, Shuang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Luyan |e verfasserin |4 aut | |
| 700 | 1 | |a Qiao, Liang |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Cheng |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Ye |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Feihong |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Tianming |e verfasserin |4 aut | |
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