Salivary Cell-Free HSD17B1 and HSPA1A Transcripts as Potential Biomarkers for Estrus Identification in Buffaloes (Bubalus Bubalis)
Abstract Background: Estrus detection is a major problem in buffaloes because of the poor expression of estrus signs leading to low reproductive efficiency. Salivary transcripts analysis is a promising tool to identify biomarker; therefore, the present study was carried out to evaluate their potential as estrus biomarker. Methods: The levels of HSD17B1, INHBA, HSPA1A, Testin transcripts were compared in saliva during estrous cycle stages [early proestrus (day -2, EP), late proestrus (day-1, LP), estrus (E), metestrus (ME) and diestrus (DE)] of cyclic heifers (n=8) and pluriparoous (n=8) buffaloes by employing quantitative real-time polymerase chain reaction (qRT-PCR). Results: The levels of HSD17B1 (EP/DE 1.46-2.43 fold, LP/DE 2.49-3.06 fold; E/DE 7.21-11.9-fold P<0.01; ME/D 1.0-1.16 fold) and HSPA1A (EP/DE 0.93-2.39 fold, LP/DE 2.68-3.23 fold; E/DE 8.52-15.18 fold P<0.01; ME/D 0.86-1.01 fold) were significantly altered during the estrus than other estrous cycle stages in both cyclic heifers and pluriparous buffaloes. Receiver operating characteristic curve analysis revealed ability of salivary HSD17B1 (AUC 0.96; P<0.001) and HSPA1A (AUC 0.99; P<0.01) to differentiate E from other stages of estrous cycle. Conclusion: Significantly higher levels of HSD17B1 and HSPA1A transcripts in saliva during estrus phase suggest their biomarkers potential for estrus detection in buffaloes..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
ResearchSquare.com - (2022) vom: 30. März Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Singha, Shubham [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.21203/rs.3.rs-1389469/v1 |
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XRA035415290 |
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| 520 | |a Abstract Background: Estrus detection is a major problem in buffaloes because of the poor expression of estrus signs leading to low reproductive efficiency. Salivary transcripts analysis is a promising tool to identify biomarker; therefore, the present study was carried out to evaluate their potential as estrus biomarker. Methods: The levels of HSD17B1, INHBA, HSPA1A, Testin transcripts were compared in saliva during estrous cycle stages [early proestrus (day -2, EP), late proestrus (day-1, LP), estrus (E), metestrus (ME) and diestrus (DE)] of cyclic heifers (n=8) and pluriparoous (n=8) buffaloes by employing quantitative real-time polymerase chain reaction (qRT-PCR). Results: The levels of HSD17B1 (EP/DE 1.46-2.43 fold, LP/DE 2.49-3.06 fold; E/DE 7.21-11.9-fold P<0.01; ME/D 1.0-1.16 fold) and HSPA1A (EP/DE 0.93-2.39 fold, LP/DE 2.68-3.23 fold; E/DE 8.52-15.18 fold P<0.01; ME/D 0.86-1.01 fold) were significantly altered during the estrus than other estrous cycle stages in both cyclic heifers and pluriparous buffaloes. Receiver operating characteristic curve analysis revealed ability of salivary HSD17B1 (AUC 0.96; P<0.001) and HSPA1A (AUC 0.99; P<0.01) to differentiate E from other stages of estrous cycle. Conclusion: Significantly higher levels of HSD17B1 and HSPA1A transcripts in saliva during estrus phase suggest their biomarkers potential for estrus detection in buffaloes. | ||
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| 700 | 1 | |a Pandey, Mamta |e verfasserin |4 aut | |
| 700 | 1 | |a Jaiswal, Latika |e verfasserin |4 aut | |
| 700 | 1 | |a Dash, Sangram |e verfasserin |4 aut | |
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| 700 | 1 | |a Mohanty, Tushar K. |e verfasserin |4 aut | |
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