Inhibitory Activity and Mechanism of Silver Nanoparticles Against Herpes Simplex Virus Type 1
Abstract Herpes simplex virus type 1 (HSV-1) is a common pathogen that infects 50-90% of the world's population and causes a variety of diseases, some of which can be life-threatening. Silver nanoparticles (AgNPs) have been proven to have broad spectrum antiviral activity. In this study, we investigated the activity of AgNPs against HSV-1 by means of some antiviral tests and transmission electron microscopy (TEM). The results of antiviral tests demonstrated that AgNPs effectively inhibited the plaque formation and progeny production of HSV-1, reduced the genomic load and interfered with the mRNA expression and the protein synthesis of HSV-1. TEM observed that AgNPs could interact with HSV-1 and deform the HSV-1 particles. Furthermore, AgNPs could affect HSV-1’s entry into cells, HSV-1’s release and cell-to-cell spread. AgNPs could also down-regulate the expression of pro-inflammatory cytokines upon HSV-1 infection. In addition, the combined treatment of AgNPs and Acyclovir (ACV) confirmed that AgNPs could significantly enhance the inhibitory effect of ACV against HSV-1. Our findings offer a new insight into clarifying the mechanism of AgNPs against HSV-1, thus providing a theoretical basis for its clinical application..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
ResearchSquare.com - (2021) vom: 03. Dez. Zur Gesamtaufnahme - year:2021 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Pan, Xuanhe [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| doi: |
10.21203/rs.3.rs-1119636/v1 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XRA034971351 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XRA034971351 | ||
| 003 | DE-627 | ||
| 005 | 20230429183345.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 220113s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.21203/rs.3.rs-1119636/v1 |2 doi | |
| 035 | |a (DE-627)XRA034971351 | ||
| 035 | |a (ResearchSquare)10.21203/rs.3.rs-1119636/v1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 570 |q DE-84 | |
| 100 | 1 | |a Pan, Xuanhe |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Inhibitory Activity and Mechanism of Silver Nanoparticles Against Herpes Simplex Virus Type 1 |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Herpes simplex virus type 1 (HSV-1) is a common pathogen that infects 50-90% of the world's population and causes a variety of diseases, some of which can be life-threatening. Silver nanoparticles (AgNPs) have been proven to have broad spectrum antiviral activity. In this study, we investigated the activity of AgNPs against HSV-1 by means of some antiviral tests and transmission electron microscopy (TEM). The results of antiviral tests demonstrated that AgNPs effectively inhibited the plaque formation and progeny production of HSV-1, reduced the genomic load and interfered with the mRNA expression and the protein synthesis of HSV-1. TEM observed that AgNPs could interact with HSV-1 and deform the HSV-1 particles. Furthermore, AgNPs could affect HSV-1’s entry into cells, HSV-1’s release and cell-to-cell spread. AgNPs could also down-regulate the expression of pro-inflammatory cytokines upon HSV-1 infection. In addition, the combined treatment of AgNPs and Acyclovir (ACV) confirmed that AgNPs could significantly enhance the inhibitory effect of ACV against HSV-1. Our findings offer a new insight into clarifying the mechanism of AgNPs against HSV-1, thus providing a theoretical basis for its clinical application. | ||
| 700 | 1 | |a Zhang, Yapeng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Yiming |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Siqi |e verfasserin |4 aut | |
| 700 | 1 | |a Guan, Chaxiang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Linqian |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Liyu |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t ResearchSquare.com |g (2021) vom: 03. Dez. |
| 773 | 1 | 8 | |g year:2021 |g day:03 |g month:12 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.21203/rs.3.rs-1119636/v1 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XRA | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |j 2021 |b 03 |c 12 | ||
| 953 | |2 045F |a 570 | ||