Neuroprotective Effects of losartan and Captopril in an H2O2- Induced Neurotoxicity Model of Neuro-2A Cells
Abstract Background: Depression is one of the most common mental illnesses and knowledge about its pathophysiology will assist in smart treatment of depression. RAS is a hormonal system that regulates blood pressure and fluid balance. Role of brain RAS has been highlighted in many mental and neurological disorders. Many drugs that target this system, such as ACEIs and ARBs, have shown positive effects on improving depression in clinical studies and animal models.Methods and results: Regarding the effectiveness RAS in depression, this study was conducted to compare the neuroprotective effects of ARB and ACEI drugs and common antidepressants on Neuro-2a cells. The cells were treated in the different concentrations of captopril, losartan, imipramine, and venlafaxine (1, 10, 50, 100 μM), after exposure to H2O2. Intracellular Ca2+ content, cell viability, SOD activity and ROS generation were measured in all groups. Our results show that cell viability of H2O2-treated cells was significantly increased in the presence of antihypertensive drugs. We observed a protective effect against ROS production in all drug groups in Neuro-2a cells. Losartan at all concentrations and captopril prevented cell damage caused by ROS. Cell death due to intracellular Ca2+, was significantly reduced with all antidepressant. At low concentrations of losartan and captopril cell death due to intracellular Ca2+was significantly reduced compared to the H2O2 group. Conclusions: Antihypertensive drugs, especially losartan can have neuroprotective effects and if approved in animal models, it may be used in the future as an adjunct in psychiatric diseases such as depression..
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Preprint| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
ResearchSquare.com - (2021) vom: 20. Dez. Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Firouzabadi, Negar [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.21203/rs.3.rs-1150729/v1 |
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XRA034952497 |
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| 520 | |a Abstract Background: Depression is one of the most common mental illnesses and knowledge about its pathophysiology will assist in smart treatment of depression. RAS is a hormonal system that regulates blood pressure and fluid balance. Role of brain RAS has been highlighted in many mental and neurological disorders. Many drugs that target this system, such as ACEIs and ARBs, have shown positive effects on improving depression in clinical studies and animal models.Methods and results: Regarding the effectiveness RAS in depression, this study was conducted to compare the neuroprotective effects of ARB and ACEI drugs and common antidepressants on Neuro-2a cells. The cells were treated in the different concentrations of captopril, losartan, imipramine, and venlafaxine (1, 10, 50, 100 μM), after exposure to H2O2. Intracellular Ca2+ content, cell viability, SOD activity and ROS generation were measured in all groups. Our results show that cell viability of H2O2-treated cells was significantly increased in the presence of antihypertensive drugs. We observed a protective effect against ROS production in all drug groups in Neuro-2a cells. Losartan at all concentrations and captopril prevented cell damage caused by ROS. Cell death due to intracellular Ca2+, was significantly reduced with all antidepressant. At low concentrations of losartan and captopril cell death due to intracellular Ca2+was significantly reduced compared to the H2O2 group. Conclusions: Antihypertensive drugs, especially losartan can have neuroprotective effects and if approved in animal models, it may be used in the future as an adjunct in psychiatric diseases such as depression. | ||
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