Droplet-based single-cell RNA sequencing of paraformaldehyde-fixed cells
Abstract Paraformaldehyde (PFA) fixation is a common fixation technique. However, current high-throughput single-cell RNA sequencing (scRNA-seq) methods are not compatible with PFA-fixed cells. Here we introduce FD-seq (Fixed Droplet RNA Sequencing), a method for droplet-based scRNA-seq of PFA-fixed, stained and sorted cells. FD-seq offers greater flexibility for researchers, and can readily be incorporated with existing Drop-seq experimental setup..
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Preprint| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
ResearchSquare.com - (2021) vom: 01. Okt. Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Phan, Hoang Van [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.21203/rs.3.pex-1604/v1 |
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| 520 | |a Abstract Paraformaldehyde (PFA) fixation is a common fixation technique. However, current high-throughput single-cell RNA sequencing (scRNA-seq) methods are not compatible with PFA-fixed cells. Here we introduce FD-seq (Fixed Droplet RNA Sequencing), a method for droplet-based scRNA-seq of PFA-fixed, stained and sorted cells. FD-seq offers greater flexibility for researchers, and can readily be incorporated with existing Drop-seq experimental setup. | ||
| 700 | 1 | |a Gent, Michiel van |e verfasserin |4 aut | |
| 700 | 1 | |a Drayman, Nir |e verfasserin |4 aut | |
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| 700 | 1 | |a Gack, Michaela U. |e verfasserin |4 aut | |
| 700 | 1 | |a Tay, Savas |e verfasserin |4 aut | |
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