Antiviral Bioactivity of Resveratrol Against Zika Virus Infection in Human Retinal Pigment Epithelial Cells
Abstract Resveratrol (RES) is a polyphenol with increasing interest for its inhibitory effects on a wide variety of viruses. Zika virus (ZIKV) is an arbovirus of the Flaviviridae family for which there is no approved treatment or vaccine, and which has become a major global health threat. Within the broad spectrum of ophthalmological manifestations after infection, retinal pigment epithelial cells (RPE) type is one of the most permissive and susceptible to the virus. This work explored the protective effects of RES on ZIKV-infected human RPE cells. RES treatment resulted in a significant reduction of infectious viral titer in infected male ARPE-19 and female hTERT-RPE1 cells. This protection was positively influenced by the action of RES on mitochondrial dynamics, restoring the ZIKV induced fragmentation of mitochondrial network to conditions similar to those of uninfected control cultures. Also, docking studies showed that RES has a high affinity for two enzymes of the rate-limiting steps of pyrimidine and purine biosynthesis and viral polymerase. In conclusion, our findings indicated that RES could be considered as an antiviral agent to treat ZIKV-induced ocular abnormalities..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
ResearchSquare.com - (2022) vom: 29. Juli Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Russo, Constanza Andrea [VerfasserIn] |
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| doi: |
10.21203/rs.3.rs-447551/v1 |
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| 520 | |a Abstract Resveratrol (RES) is a polyphenol with increasing interest for its inhibitory effects on a wide variety of viruses. Zika virus (ZIKV) is an arbovirus of the Flaviviridae family for which there is no approved treatment or vaccine, and which has become a major global health threat. Within the broad spectrum of ophthalmological manifestations after infection, retinal pigment epithelial cells (RPE) type is one of the most permissive and susceptible to the virus. This work explored the protective effects of RES on ZIKV-infected human RPE cells. RES treatment resulted in a significant reduction of infectious viral titer in infected male ARPE-19 and female hTERT-RPE1 cells. This protection was positively influenced by the action of RES on mitochondrial dynamics, restoring the ZIKV induced fragmentation of mitochondrial network to conditions similar to those of uninfected control cultures. Also, docking studies showed that RES has a high affinity for two enzymes of the rate-limiting steps of pyrimidine and purine biosynthesis and viral polymerase. In conclusion, our findings indicated that RES could be considered as an antiviral agent to treat ZIKV-induced ocular abnormalities. | ||
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