Effects of perioperative managements on ocular surface microbiota in intravitreal injection patients
Abstract Background: We investigated the effects of perioperative managements on ocular surface microbiota in patients who received intravitreal injections.Methods: Samples of ocular surface microbiota were obtained from 41 eyes of 41 patients who received intravitreal injections at the First Affiliated Hospital of Xi'an Jiaotong University and People’s Hospital in Shaanxi Province. The microbial 16s rDNA from samples were analyzed using an Illumina MiSeq high-throughput sequencing platform. Operating classification units (OTU) clustering and alpha/beta diversity analysis was performed for species classification.Results: High-throughput sequencing yielded 1697337 sequences and 1396, indicating an equivalent number of ocular surface microbiota. OTU was significantly lower in the eyes that received intravitreal injections, compared with those who did not received injections (P<0.05). The number of OTUs was inversely correlated with the administered of perioperative managements and the times of intravitreal injections. Beta diversity showed a significant difference among each groups (P<0.05). With the increased perioperative managements, the prevalence of gram-positive bacteria (Clostridium) had decreased and the prevalence of gram-negative bacteria (Neisseria) had increased.Conclusions: Microbiota on the ocular surface were significantly different between treated and control eyes. And the composition of microbiota on the ocular surface was dramatically changed over time in eyes that received intravitreal injections. Perioperative managements might disrupt the balance of the ocular surface microbiota, increasing risk of infectious disease. Therefore, perioperative managements including local antibiotic eye drops should be applied cautiously before intraocular surgery..
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Preprint| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
ResearchSquare.com - (2021) vom: 17. März Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hu, Yaguang [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.21203/rs.3.rs-154655/v1 |
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| PPN (Katalog-ID): |
XRA03369592X |
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| 520 | |a Abstract Background: We investigated the effects of perioperative managements on ocular surface microbiota in patients who received intravitreal injections.Methods: Samples of ocular surface microbiota were obtained from 41 eyes of 41 patients who received intravitreal injections at the First Affiliated Hospital of Xi'an Jiaotong University and People’s Hospital in Shaanxi Province. The microbial 16s rDNA from samples were analyzed using an Illumina MiSeq high-throughput sequencing platform. Operating classification units (OTU) clustering and alpha/beta diversity analysis was performed for species classification.Results: High-throughput sequencing yielded 1697337 sequences and 1396, indicating an equivalent number of ocular surface microbiota. OTU was significantly lower in the eyes that received intravitreal injections, compared with those who did not received injections (P<0.05). The number of OTUs was inversely correlated with the administered of perioperative managements and the times of intravitreal injections. Beta diversity showed a significant difference among each groups (P<0.05). With the increased perioperative managements, the prevalence of gram-positive bacteria (Clostridium) had decreased and the prevalence of gram-negative bacteria (Neisseria) had increased.Conclusions: Microbiota on the ocular surface were significantly different between treated and control eyes. And the composition of microbiota on the ocular surface was dramatically changed over time in eyes that received intravitreal injections. Perioperative managements might disrupt the balance of the ocular surface microbiota, increasing risk of infectious disease. Therefore, perioperative managements including local antibiotic eye drops should be applied cautiously before intraocular surgery. | ||
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