A coumarin-based array to discriminate between different types of amyloid fibrils
Self-assembly of misfolded proteins can lead to the formation of amyloids, which are implicated in the onset of many pathologies including Alzheimer’s disease and Parkinson’s disease. The facile detection and discrimination of different amyloids is crucial for early diagnosis of amyloid-related pathologies. Here, we report the development of a fluorescent two-sensor array which is able to correctly discriminate between four different amyloids implicated in amyloid-related pathologies with 100% classification. The array was also applied to mouse models of Alzheimer’s disease and was able to discriminate between samples from mice corresponding to early (6 months) and advanced (12 months) stages of Alzheimer’s disease. Finally, the flexibility of the array was assessed by expanding the analytes to include functional amyloids. The same two-sensor array was able to correctly discriminate between eight different disease-associated and functional amyloids with 100% classification..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
chemRxiv.org - (2023) vom: 26. Juni Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Trinh, Natalie [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
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| doi: |
10.26434/chemrxiv-2023-sq6gz |
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| 520 | |a Self-assembly of misfolded proteins can lead to the formation of amyloids, which are implicated in the onset of many pathologies including Alzheimer’s disease and Parkinson’s disease. The facile detection and discrimination of different amyloids is crucial for early diagnosis of amyloid-related pathologies. Here, we report the development of a fluorescent two-sensor array which is able to correctly discriminate between four different amyloids implicated in amyloid-related pathologies with 100% classification. The array was also applied to mouse models of Alzheimer’s disease and was able to discriminate between samples from mice corresponding to early (6 months) and advanced (12 months) stages of Alzheimer’s disease. Finally, the flexibility of the array was assessed by expanding the analytes to include functional amyloids. The same two-sensor array was able to correctly discriminate between eight different disease-associated and functional amyloids with 100% classification. | ||
| 650 | 4 | |a Chemistry |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Bhuskute, Kaustubh R. |4 aut | |
| 700 | 1 | |a Varghese, Nikhil R. |4 aut | |
| 700 | 1 | |a Buchanan, Jessica A. |4 aut | |
| 700 | 1 | |a McCutcheon, Fiona M. |4 aut | |
| 700 | 1 | |a Medcalf, Robert L. |4 aut | |
| 700 | 1 | |a Jolliffe, Katrina A. |4 aut | |
| 700 | 1 | |a Sunde, Margaret |4 aut | |
| 700 | 1 | |a New, Elizabeth J. |4 aut | |
| 700 | 1 | |a Kaur, Amandeep |0 (orcid)0000-0002-0898-875X |4 aut | |
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