A Rapid and Quantitative Serum Test for SARS-CoV-2 Antibodies with Portable Surface Plasmon Resonance Sensing
We report a surface plasmon resonance (SPR) sensor detecting nucleocapsid antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in undiluted human serum. When exposed to SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the patient population immunized against SARD-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with SARS-CoV-2 nucleocapsid recombinant protein detected anti-SARS-CoV-2 antibodies in the nanomolar range. This bioassay was performed on a portable SPR instrument in undiluted human serum and results were collected within 15 minutes of sample/sensor contact. This strategy paves the way to point-of-care and label-free rapid testing for antibodies..
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Preprint| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
chemRxiv.org - (2021) vom: 17. Nov. Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Djaileb, Abdelhadi [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.26434/chemrxiv.12118914 |
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| 520 | |a We report a surface plasmon resonance (SPR) sensor detecting nucleocapsid antibodies specific against the novel coronavirus 2019 (SARS-CoV-2) in undiluted human serum. When exposed to SARS-CoV-2, the immune system responds by expressing antibodies at levels that can be detected and monitored to identify the patient population immunized against SARD-CoV-2 and support efforts to deploy a vaccine strategically. A SPR sensor coated with a peptide monolayer and functionalized with SARS-CoV-2 nucleocapsid recombinant protein detected anti-SARS-CoV-2 antibodies in the nanomolar range. This bioassay was performed on a portable SPR instrument in undiluted human serum and results were collected within 15 minutes of sample/sensor contact. This strategy paves the way to point-of-care and label-free rapid testing for antibodies. | ||
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| 700 | 1 | |a Charron, Benjamin |e verfasserin |4 aut | |
| 700 | 1 | |a Jodaylami, Maryam Hojjat |e verfasserin |4 aut | |
| 700 | 1 | |a Thibault, Vincent |e verfasserin |4 aut | |
| 700 | 1 | |a Coutu, Julien |e verfasserin |4 aut | |
| 700 | 1 | |a Stevenson, Keisean |e verfasserin |4 aut | |
| 700 | 1 | |a Forest, Simon |e verfasserin |4 aut | |
| 700 | 1 | |a Live, Ludovic S. |e verfasserin |4 aut | |
| 700 | 1 | |a Boudreau, Denis |e verfasserin |4 aut | |
| 700 | 1 | |a Pelletier, Joelle N. |e verfasserin |4 aut | |
| 700 | 1 | |a Masson, Jean-Francois |e verfasserin |4 aut | |
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