A framework for quality control in quantitative proteomics
Abstract A thorough evaluation of the quality, reproducibility, and variability of bottom-up proteomics data is necessary at every stage of a workflow from planning to analysis. We share real-world case studies applying adaptable quality control (QC) measures to assess sample preparation, system function, and quantitative analysis. System suitability samples are repeatedly measured longitudinally with targeted methods, and we share examples where they are used on three instrument platforms to identify severe system failures and track function over months to years. Internal QCs incorporated at protein and peptide-level allow our team to assess sample preparation issues and to differentiate system failures from sample-specific issues. External QC samples prepared alongside our experimental samples are used to verify the consistency and quantitative potential of our results during batch correction and normalization before assessing biological phenotypes. We combine these controls with rapid analysis using Skyline, longitudinal QC metrics using AutoQC, and server-based data deposition using PanoramaWeb. We propose that this integrated approach to QC be used as a starting point for groups to facilitate rapid quality control assessment to ensure that valuable instrument time is used to collect the best quality data possible..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 29. Apr. Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Tsantilas, Kristine A. [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| Themen: |
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| doi: |
10.1101/2024.04.12.589318 |
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XBI043256996 |
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| 520 | |a Abstract A thorough evaluation of the quality, reproducibility, and variability of bottom-up proteomics data is necessary at every stage of a workflow from planning to analysis. We share real-world case studies applying adaptable quality control (QC) measures to assess sample preparation, system function, and quantitative analysis. System suitability samples are repeatedly measured longitudinally with targeted methods, and we share examples where they are used on three instrument platforms to identify severe system failures and track function over months to years. Internal QCs incorporated at protein and peptide-level allow our team to assess sample preparation issues and to differentiate system failures from sample-specific issues. External QC samples prepared alongside our experimental samples are used to verify the consistency and quantitative potential of our results during batch correction and normalization before assessing biological phenotypes. We combine these controls with rapid analysis using Skyline, longitudinal QC metrics using AutoQC, and server-based data deposition using PanoramaWeb. We propose that this integrated approach to QC be used as a starting point for groups to facilitate rapid quality control assessment to ensure that valuable instrument time is used to collect the best quality data possible. | ||
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| 700 | 1 | |a Merrihew, Gennifer E. |e verfasserin |0 (orcid)0000-0003-4903-0318 |4 aut | |
| 700 | 1 | |a Robbins, Julia E. |e verfasserin |4 aut | |
| 700 | 1 | |a Johnson, Richard S. |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Jea |e verfasserin |4 aut | |
| 700 | 1 | |a Plubell, Deanna L. |e verfasserin |0 (orcid)0000-0002-6580-8073 |4 aut | |
| 700 | 1 | |a Huang, Eric |e verfasserin |4 aut | |
| 700 | 1 | |a Riffle, Michael |e verfasserin |0 (orcid)0000-0003-1633-8607 |4 aut | |
| 700 | 1 | |a Sharma, Vagisha |e verfasserin |0 (orcid)0000-0003-1922-439X |4 aut | |
| 700 | 1 | |a MacLean, Brendan X. |e verfasserin |0 (orcid)0000-0002-9575-0255 |4 aut | |
| 700 | 1 | |a Eckels, Josh |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Christine C. |e verfasserin |4 aut | |
| 700 | 1 | |a Bereman, Michael S. |e verfasserin |0 (orcid)0000-0002-2722-064X |4 aut | |
| 700 | 1 | |a Spencer, Sandra E. |e verfasserin |0 (orcid)0000-0001-6446-1584 |4 aut | |
| 700 | 1 | |a Hoofnagle, Andrew N. |e verfasserin |0 (orcid)0000-0002-6449-0243 |4 aut | |
| 700 | 1 | |a MacCoss, Michael J. |e verfasserin |0 (orcid)0000-0003-1853-0256 |4 aut | |
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