MOS4-Associated Complex subunits 3A and 3B modulate<i>FLM</i>splicing to repress photoperiod-dependent floral transition
Abstract Plants adjust their flowering time by integrating environmental cues through complex regulatory networks. RNA splicing plays a crucial role in modulating gene expression in response to flowering signals. The MOS4-associated complex (MAC), consisting of the evolutionarily conserved E3 ubiquitin ligases MAC3A and MAC3B, is pivotal in splicing regulation. However, their involvement in floral transition remained unclear. This study observed thatmac3a/mac3bmutants flowered significantly earlier under short-day (SD) conditions, a phenotype absent under long-day (LD) conditions. This early flowering correlated with upregulation ofFLOWERING LOCUS T(FT) andSUPPRESSOR OF OVEREXPRESSION OF CO 1(SOC1) compared to wild-type plants. Transcriptomic analysis revealed alterations in transcript levels and splicing profiles of key floral regulators across different flowering pathways. Further exploration identified the thermosensory flowering regulatorFLOWERING LOCUS M(FLM) as being influenced byMAC3AandMAC3B. Our findings showed that SPL9 bound toMAC3AandMAC3Bpromoters to induce their expression under a short photoperiod. Subsequently, MAC3A and MAC3B associated withFLMtranscripts to modulate their splicing in SD. This study elucidates how the MAC complex, through RNA splicing regulation, integrates environmental signals to modulate flowering, unveiling a new layer of complexity in flowering pathway crosstalk under non-inductive photoperiods..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 29. März Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Yu-Wen [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2024.03.26.586198 |
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| PPN (Katalog-ID): |
XBI043072666 |
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| 100 | 1 | |a Huang, Yu-Wen |e verfasserin |0 (orcid)0009-0007-6267-8895 |4 aut | |
| 245 | 1 | 0 | |a MOS4-Associated Complex subunits 3A and 3B modulate<i>FLM</i>splicing to repress photoperiod-dependent floral transition |
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| 520 | |a Abstract Plants adjust their flowering time by integrating environmental cues through complex regulatory networks. RNA splicing plays a crucial role in modulating gene expression in response to flowering signals. The MOS4-associated complex (MAC), consisting of the evolutionarily conserved E3 ubiquitin ligases MAC3A and MAC3B, is pivotal in splicing regulation. However, their involvement in floral transition remained unclear. This study observed thatmac3a/mac3bmutants flowered significantly earlier under short-day (SD) conditions, a phenotype absent under long-day (LD) conditions. This early flowering correlated with upregulation ofFLOWERING LOCUS T(FT) andSUPPRESSOR OF OVEREXPRESSION OF CO 1(SOC1) compared to wild-type plants. Transcriptomic analysis revealed alterations in transcript levels and splicing profiles of key floral regulators across different flowering pathways. Further exploration identified the thermosensory flowering regulatorFLOWERING LOCUS M(FLM) as being influenced byMAC3AandMAC3B. Our findings showed that SPL9 bound toMAC3AandMAC3Bpromoters to induce their expression under a short photoperiod. Subsequently, MAC3A and MAC3B associated withFLMtranscripts to modulate their splicing in SD. This study elucidates how the MAC complex, through RNA splicing regulation, integrates environmental signals to modulate flowering, unveiling a new layer of complexity in flowering pathway crosstalk under non-inductive photoperiods. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Tseng, Chih-Yen |0 (orcid)0009-0004-1339-5213 |4 aut | |
| 700 | 1 | |a Tu, Yi-Tsung |4 aut | |
| 700 | 1 | |a Hsieh, Hsin-Yu |0 (orcid)0000-0001-8457-6872 |4 aut | |
| 700 | 1 | |a Wang, Yu-Sen |4 aut | |
| 700 | 1 | |a Chen, Yu-Zhen |4 aut | |
| 700 | 1 | |a Ly, Yun-Tung |4 aut | |
| 700 | 1 | |a Tu, Shih-Long |0 (orcid)0000-0001-9436-278X |4 aut | |
| 700 | 1 | |a Lee, Chin-Mei |0 (orcid)0000-0003-3870-4268 |4 aut | |
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