SARS-CoV-2 infection activates inflammatory macrophages in vascular immune organoids
SARS-CoV-2 provokes devastating tissue damage by cytokine release syndrome and leads to multi-organ failure. Modeling the process of immune cell activation and subsequent tissue damage is a significant task. Organoids from human tissues advanced our understanding of SARS-CoV-2 infection mechanisms though, they are missing crucial components: immune cells and endothelial cells. This study aims to generate organoids with these components. We established vascular immune organoids from human pluripotent stem cells and examined the effect of SARS-CoV-2 infection. We demonstrated that infections activated inflammatory macrophages. Notably, the upregulation of interferon signaling supports macrophages role in cytokine release syndrome. We propose vascular immune organoids are a useful platform to model and discover factors that ameliorate SARS-CoV-2-mediated cytokine release syndrome..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 22. März Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chau, Chiu Wang [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2024.03.20.585837 |
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| PPN (Katalog-ID): |
XBI043010032 |
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| 520 | |a SARS-CoV-2 provokes devastating tissue damage by cytokine release syndrome and leads to multi-organ failure. Modeling the process of immune cell activation and subsequent tissue damage is a significant task. Organoids from human tissues advanced our understanding of SARS-CoV-2 infection mechanisms though, they are missing crucial components: immune cells and endothelial cells. This study aims to generate organoids with these components. We established vascular immune organoids from human pluripotent stem cells and examined the effect of SARS-CoV-2 infection. We demonstrated that infections activated inflammatory macrophages. Notably, the upregulation of interferon signaling supports macrophages role in cytokine release syndrome. We propose vascular immune organoids are a useful platform to model and discover factors that ameliorate SARS-CoV-2-mediated cytokine release syndrome. | ||
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| 700 | 1 | |a Au-Yeung, Rex K.H. |4 aut | |
| 700 | 1 | |a Tang, Kaiming |4 aut | |
| 700 | 1 | |a Xiang, Yang |4 aut | |
| 700 | 1 | |a Ruan, Degong |4 aut | |
| 700 | 1 | |a Zhang, Lanlan |4 aut | |
| 700 | 1 | |a Wong, Hera |4 aut | |
| 700 | 1 | |a Zhang, Shihui |4 aut | |
| 700 | 1 | |a Au, Man Ting |4 aut | |
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| 700 | 1 | |a Song, Euijeong |4 aut | |
| 700 | 1 | |a Choi, Dong-Hee |4 aut | |
| 700 | 1 | |a Liu, Pentao |4 aut | |
| 700 | 1 | |a Yuan, Shuofeng |4 aut | |
| 700 | 1 | |a Wen, Chunyi |4 aut | |
| 700 | 1 | |a Sugimura, Ryohichi |4 aut | |
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