Thymic epithelial organoids mediate T cell development
Abstract Although the advent of organoids opened unprecedented perspectives for basic and translational research, immune system-related organoids remain largely underdeveloped. Here we established organoids from the thymus, the lymphoid organ responsible for T cell development. We identified conditions enabling thymic epithelial progenitor cell proliferation and development into organoids with in vivo-like transcriptional profiles and diverse cell populations. Contrary to two-dimensional cultures, thymic epithelial organoids maintained thymus functionality in vitro and mediated physiological T cell development upon reaggregation with T cell progenitors. The reaggregates showed in vivo-like epithelial diversity and ability to attract T cell progenitors. Thymic epithelial organoids provide new opportunities to study TEC biology and T cell development in vitro, pave the way for future thymic regeneration strategies and are the first organoids originating from the stromal compartment of a lymphoid organ.Summary statement Establishment of organoids from the epithelial cells of the thymus which resemble their in vivo counterpart and have thymopoietic ability in reaggregate culture..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 14. März Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hübscher, Tania [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2024.03.05.583513 |
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| PPN (Katalog-ID): |
XBI042831954 |
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| 245 | 1 | 0 | |a Thymic epithelial organoids mediate T cell development |
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| 520 | |a Abstract Although the advent of organoids opened unprecedented perspectives for basic and translational research, immune system-related organoids remain largely underdeveloped. Here we established organoids from the thymus, the lymphoid organ responsible for T cell development. We identified conditions enabling thymic epithelial progenitor cell proliferation and development into organoids with in vivo-like transcriptional profiles and diverse cell populations. Contrary to two-dimensional cultures, thymic epithelial organoids maintained thymus functionality in vitro and mediated physiological T cell development upon reaggregation with T cell progenitors. The reaggregates showed in vivo-like epithelial diversity and ability to attract T cell progenitors. Thymic epithelial organoids provide new opportunities to study TEC biology and T cell development in vitro, pave the way for future thymic regeneration strategies and are the first organoids originating from the stromal compartment of a lymphoid organ.Summary statement Establishment of organoids from the epithelial cells of the thymus which resemble their in vivo counterpart and have thymopoietic ability in reaggregate culture. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Lorenzo-Martín, L. Francisco |4 aut | |
| 700 | 1 | |a Barthlott, Thomas |4 aut | |
| 700 | 1 | |a Tillard, Lucie |4 aut | |
| 700 | 1 | |a Langer, Jakob J. |4 aut | |
| 700 | 1 | |a Rouse, Paul |4 aut | |
| 700 | 1 | |a Blackburn, C. Clare |4 aut | |
| 700 | 1 | |a Holländer, Georg |4 aut | |
| 700 | 1 | |a Lutolf, Matthias P. |0 (orcid)0000-0002-5898-305X |4 aut | |
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