Liquid phase electron microscopy of bacterial ultrastructure
Abstract Recent advances in liquid phase scanning transmission electron microscopy (LP-STEM) have enabled the study of dynamic biological processes at nanometre resolutions, paving the way for live-cell imaging using electron microscopy. However, this technique is often hampered by the inherent thickness of whole cell samples and damage from electron beam irradiation. These restrictions degrade image quality and resolution, impeding biological interpretation. Here we detail the use of graphene encapsulation, STEM, and energy-dispersive X-ray (EDX) spectroscopy methods to mitigate these issues, providing unprecedented levels of intracellular detail in aqueous specimens. This work demonstrates the potential of LP-STEM to examine and identify internal cellular structures in thick biological samples, in a radiation resistant, gram-positive bacterium, Deinococcus radiodurans using a variety of imaging techniques..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 28. März Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Caffrey, Brian J. [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2024.02.27.580996 |
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| PPN (Katalog-ID): |
XBI042676487 |
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| 520 | |a Abstract Recent advances in liquid phase scanning transmission electron microscopy (LP-STEM) have enabled the study of dynamic biological processes at nanometre resolutions, paving the way for live-cell imaging using electron microscopy. However, this technique is often hampered by the inherent thickness of whole cell samples and damage from electron beam irradiation. These restrictions degrade image quality and resolution, impeding biological interpretation. Here we detail the use of graphene encapsulation, STEM, and energy-dispersive X-ray (EDX) spectroscopy methods to mitigate these issues, providing unprecedented levels of intracellular detail in aqueous specimens. This work demonstrates the potential of LP-STEM to examine and identify internal cellular structures in thick biological samples, in a radiation resistant, gram-positive bacterium, Deinococcus radiodurans using a variety of imaging techniques. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Pedrazo-Tardajos, Adrián |0 (orcid)0000-0003-4181-9226 |4 aut | |
| 700 | 1 | |a Liberti, Emanuela |0 (orcid)0000-0001-5306-7936 |4 aut | |
| 700 | 1 | |a Gaunt, Ben |4 aut | |
| 700 | 1 | |a Kim, Judy S. |0 (orcid)0000-0003-1513-9964 |4 aut | |
| 700 | 1 | |a Kirkland, Angus I. |0 (orcid)0000-0001-8068-1990 |4 aut | |
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