Parasite contingency loci and the evolution of host specificity: Simple sequence repeats mediate<i>Bartonella</i>adaptation to a wild rodent host
Abstract Parasites can adapt to better exploit their hosts on many timescales, ranging from within a single infection to across serial infections of multiple hosts. However, little is known about how the genomes of parasites in natural communities evolve when they face diverse hosts. We investigated howBartonellabacteria that circulate in rodent communities in the dunes of the Negev Desert in Israel adapt to different hosts. We propagated 15Bartonellapopulations through infections of either a single rodent species (Gerbillus andersoniorGerbillus pyramidum) or alternating between the two. After 20 rodent passages, strains withde novomutations outcompeted the ancestor in most lines. Mutations in two mononucleotide simple sequence repeats (SSRs) dominated the evolutionary dynamics and caused frameshifts in the same adhesin gene. These mutations appeared exclusively in populations that encounteredG. andersoni, and they improved the ability to exploit this host. Similar SSRs in other genes are conserved and exhibit ON/OFF variation inBartonellaisolates from the Negev Desert dunes. Our results suggest that SSR-based contingency loci are important not only for rapidly and reversibly generating antigenic variation to escape immune responses but may also play a prominent role in the evolution of host specificity..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 25. Jan. Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rodríguez‑Pastor, Ruth [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2024.01.18.576196 |
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| PPN (Katalog-ID): |
XBI042244366 |
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| 100 | 1 | |a Rodríguez‑Pastor, Ruth |e verfasserin |0 (orcid)0000-0001-8435-6386 |4 aut | |
| 245 | 1 | 0 | |a Parasite contingency loci and the evolution of host specificity: Simple sequence repeats mediate<i>Bartonella</i>adaptation to a wild rodent host |
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| 520 | |a Abstract Parasites can adapt to better exploit their hosts on many timescales, ranging from within a single infection to across serial infections of multiple hosts. However, little is known about how the genomes of parasites in natural communities evolve when they face diverse hosts. We investigated howBartonellabacteria that circulate in rodent communities in the dunes of the Negev Desert in Israel adapt to different hosts. We propagated 15Bartonellapopulations through infections of either a single rodent species (Gerbillus andersoniorGerbillus pyramidum) or alternating between the two. After 20 rodent passages, strains withde novomutations outcompeted the ancestor in most lines. Mutations in two mononucleotide simple sequence repeats (SSRs) dominated the evolutionary dynamics and caused frameshifts in the same adhesin gene. These mutations appeared exclusively in populations that encounteredG. andersoni, and they improved the ability to exploit this host. Similar SSRs in other genes are conserved and exhibit ON/OFF variation inBartonellaisolates from the Negev Desert dunes. Our results suggest that SSR-based contingency loci are important not only for rapidly and reversibly generating antigenic variation to escape immune responses but may also play a prominent role in the evolution of host specificity. | ||
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| 700 | 1 | |a Knossow, Nadav |0 (orcid)0000-0002-0578-8883 |4 aut | |
| 700 | 1 | |a Shahar, Naama |4 aut | |
| 700 | 1 | |a Hasik, Adam Z. |0 (orcid)0000-0002-4069-7186 |4 aut | |
| 700 | 1 | |a Deatherage, Daniel E. |0 (orcid)0000-0001-8914-8359 |4 aut | |
| 700 | 1 | |a Gutiérrez, Ricardo |4 aut | |
| 700 | 1 | |a Harrus, Shimon |0 (orcid)0000-0003-0542-207X |4 aut | |
| 700 | 1 | |a Zaman, Luis |0 (orcid)0000-0001-6838-7385 |4 aut | |
| 700 | 1 | |a Lenski, Richard E. |0 (orcid)0000-0002-1064-8375 |4 aut | |
| 700 | 1 | |a Barrick, Jeffrey E. |0 (orcid)0000-0003-0888-7358 |4 aut | |
| 700 | 1 | |a Hawlena, Hadas |0 (orcid)0000-0002-0634-2920 |4 aut | |
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