Liver Protects Cytoarchitecture, Neuron Viability and Electrocortical Activity in Post-Cardiac Arrest Brain Injury
ABSTRACT BACKGROUND Brain injury is the major reason for patient deaths in victims who survive after cardiac arrest. Clinical studies have shown that the presence of hypoxic hepatitis and pre-cardiac arrest liver disease is associated with increased mortality and inferior neurological recovery. However, how the liver might impact the pathogenesis of post-cardiac arrest is still unknown.METHODS Anin vivoglobal cerebral ischemia model was established to assess how simultaneous liver ischemia affected the recovery of brain ischemic injury. In addition, anex vivobrain normothermic machine perfusion (NMP) model was established to evaluate how addition of a functioning liver might impact the circulation, cytoarchitecture, neuron viability and electrocortical activity of the reperfused brain post-cardiac arrest.RESULTS In thein vivomodel, we observed a larger infarct area in the frontal lobe, elevated tissue injury scores in the CA1 region, as well as increased intravascular immune cell adhesion in the reperfused brains with hepatic ischemia, compared to those without simultaneous hepatic ischemia. The results of theex vivomodel demonstrated that the addition of a functioning liver to the brain NMP circuit significantly reduced post-cardiac arrest brain injury, increased neuronal viability and improved electrocortical activity. Furthermore, we observed significant alterations in gene expressions and metabolites in the presence or absence of hepatic ischemia.CONCLUSIONS Our research highlights the crucial role of the liver in the pathogenesis of post-cardiac arrest brain injury. These findings shed lights on a cardio-pulmonary-hepatic brain resuscitation strategy for patients with cardiac arrest..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 19. Dez. Zur Gesamtaufnahme - year:2023 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Guo, Zhiyong [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| doi: |
10.1101/2023.12.13.571604 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI041878108 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI041878108 | ||
| 003 | DE-627 | ||
| 005 | 20231220101558.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231215s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2023.12.13.571604 |2 doi | |
| 035 | |a (DE-627)XBI041878108 | ||
| 035 | |a (biorXiv)10.1101/2023.12.13.571604 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Guo, Zhiyong |e verfasserin |0 (orcid)0000-0002-5458-6268 |4 aut | |
| 245 | 1 | 0 | |a Liver Protects Cytoarchitecture, Neuron Viability and Electrocortical Activity in Post-Cardiac Arrest Brain Injury |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a ABSTRACT BACKGROUND Brain injury is the major reason for patient deaths in victims who survive after cardiac arrest. Clinical studies have shown that the presence of hypoxic hepatitis and pre-cardiac arrest liver disease is associated with increased mortality and inferior neurological recovery. However, how the liver might impact the pathogenesis of post-cardiac arrest is still unknown.METHODS Anin vivoglobal cerebral ischemia model was established to assess how simultaneous liver ischemia affected the recovery of brain ischemic injury. In addition, anex vivobrain normothermic machine perfusion (NMP) model was established to evaluate how addition of a functioning liver might impact the circulation, cytoarchitecture, neuron viability and electrocortical activity of the reperfused brain post-cardiac arrest.RESULTS In thein vivomodel, we observed a larger infarct area in the frontal lobe, elevated tissue injury scores in the CA1 region, as well as increased intravascular immune cell adhesion in the reperfused brains with hepatic ischemia, compared to those without simultaneous hepatic ischemia. The results of theex vivomodel demonstrated that the addition of a functioning liver to the brain NMP circuit significantly reduced post-cardiac arrest brain injury, increased neuronal viability and improved electrocortical activity. Furthermore, we observed significant alterations in gene expressions and metabolites in the presence or absence of hepatic ischemia.CONCLUSIONS Our research highlights the crucial role of the liver in the pathogenesis of post-cardiac arrest brain injury. These findings shed lights on a cardio-pulmonary-hepatic brain resuscitation strategy for patients with cardiac arrest. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Yin, Meixian |0 (orcid)0000-0001-8525-7603 |4 aut | |
| 700 | 1 | |a Sun, Chengjun |4 aut | |
| 700 | 1 | |a Xu, Guixing |4 aut | |
| 700 | 1 | |a Wang, Tielong |4 aut | |
| 700 | 1 | |a Jia, Zehua |4 aut | |
| 700 | 1 | |a Zhang, Zhiheng |4 aut | |
| 700 | 1 | |a Zhu, Caihui |4 aut | |
| 700 | 1 | |a Zheng, Donghua |4 aut | |
| 700 | 1 | |a Wang, Linhe |4 aut | |
| 700 | 1 | |a Huang, Shanzhou |4 aut | |
| 700 | 1 | |a Liu, Di |4 aut | |
| 700 | 1 | |a Zhang, Yixi |4 aut | |
| 700 | 1 | |a Xie, Rongxing |4 aut | |
| 700 | 1 | |a Gao, Ningxin |4 aut | |
| 700 | 1 | |a Yu, Ping |4 aut | |
| 700 | 1 | |a Zhan, Liqiang |4 aut | |
| 700 | 1 | |a He, Shujiao |4 aut | |
| 700 | 1 | |a Zhu, Yifan |4 aut | |
| 700 | 1 | |a Li, Yuexin |4 aut | |
| 700 | 1 | |a Nashan, Björn |4 aut | |
| 700 | 1 | |a Andrea, Schlegel |4 aut | |
| 700 | 1 | |a Xu, Jin |4 aut | |
| 700 | 1 | |a Zhao, Qiang |4 aut | |
| 700 | 1 | |a He, Xiaoshun |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2023) vom: 19. Dez. |
| 773 | 1 | 8 | |g year:2023 |g day:19 |g month:12 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2023.12.13.571604 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 19 |c 12 | ||