Temporal dynamics of skin microbiota and immune correlates in psoriasis patients receiving systemic treatment
ABSTRACT <jats:sec id="s45">Background How skin microbiota in psoriasis patients responded to systematic therapeutics remained unknown.<jats:sec id="s46">Objectives To profile temporal shifts in transcriptionally active skin microbiota in psoriasis patients receiving systemic therapies.<jats:sec id="s47">Methods We prospectively enrolled 61 psoriasis patients and 29 skin-healthy controls in 2015-2019. Using RNA-based 16S rRNA gene sequencing, we analyzed 969 samples from skin lesions and compared microbial abundance and diversity by therapeutic classes and disease severity.<jats:sec id="s48">Results Lesional microbiota in patients on conventional systemics and TNF-αinhibitor was different in relative abundances in Firmicutes (7.83% higher, adjusted P < 0.001) and Proteobacteria (6.98% lower, adjusted P < 0.01) from that in patients on anti-interleukin monoclonal antibodies (anti-ILAb) at baseline. The only difference during treatment was a 1.47% lower abundance in Bacteroides associated with nonbiologics use (adjusted P < 0.01). We identified no indicator taxa by disease severity at baseline yet noticed that a minor relative reduction inCorynebacteriumsp. was associated with clinical responses to treatment.Compared to anti-ILAb, TNF-αinhibitor and nonbiologics were associated with -0.21 lower Shannon Diversity (adjusted P < 0.01) and 0.03 higher Shannon Evenness (adjusted P < 0.01). Results of ordinated principal coordinates analysis revealed that, lesional microbiota from patients of these 3 therapeutic groups was compositionally distinct. Our work also demonstrated concurrent changes in clonal shifts in systemic T cell receptor clonotypes that were associated with systemic use of biologics.<jats:sec id="s49">Conclusions Community abundances and diversities of skin microbiota may be useful in distinguishing skin microbiota from patients receiving different systemic therapeutics. Specifically, use of anti-ILAb and TNF-αinhibitor was associated with sample-wise microbial abundances and diversities, but not richness, over time. These findings highlighted the potential utility of skin microbiota as biomarkers for personalized treatment plans in patients with moderate-to-severe psoriasis..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 05. Dez. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Su-Hsun [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2023.11.27.23298999 |
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| PPN (Katalog-ID): |
XBI041683714 |
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| 520 | |a ABSTRACT <jats:sec id="s45">Background How skin microbiota in psoriasis patients responded to systematic therapeutics remained unknown.<jats:sec id="s46">Objectives To profile temporal shifts in transcriptionally active skin microbiota in psoriasis patients receiving systemic therapies.<jats:sec id="s47">Methods We prospectively enrolled 61 psoriasis patients and 29 skin-healthy controls in 2015-2019. Using RNA-based 16S rRNA gene sequencing, we analyzed 969 samples from skin lesions and compared microbial abundance and diversity by therapeutic classes and disease severity.<jats:sec id="s48">Results Lesional microbiota in patients on conventional systemics and TNF-αinhibitor was different in relative abundances in Firmicutes (7.83% higher, adjusted P < 0.001) and Proteobacteria (6.98% lower, adjusted P < 0.01) from that in patients on anti-interleukin monoclonal antibodies (anti-ILAb) at baseline. The only difference during treatment was a 1.47% lower abundance in Bacteroides associated with nonbiologics use (adjusted P < 0.01). We identified no indicator taxa by disease severity at baseline yet noticed that a minor relative reduction inCorynebacteriumsp. was associated with clinical responses to treatment.Compared to anti-ILAb, TNF-αinhibitor and nonbiologics were associated with -0.21 lower Shannon Diversity (adjusted P < 0.01) and 0.03 higher Shannon Evenness (adjusted P < 0.01). Results of ordinated principal coordinates analysis revealed that, lesional microbiota from patients of these 3 therapeutic groups was compositionally distinct. Our work also demonstrated concurrent changes in clonal shifts in systemic T cell receptor clonotypes that were associated with systemic use of biologics.<jats:sec id="s49">Conclusions Community abundances and diversities of skin microbiota may be useful in distinguishing skin microbiota from patients receiving different systemic therapeutics. Specifically, use of anti-ILAb and TNF-αinhibitor was associated with sample-wise microbial abundances and diversities, but not richness, over time. These findings highlighted the potential utility of skin microbiota as biomarkers for personalized treatment plans in patients with moderate-to-severe psoriasis. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Huang, Yu-Huei |4 aut | |
| 700 | 1 | |a Weng, Hao-Jui |4 aut | |
| 700 | 1 | |a Tsai, Tsen-Fang |4 aut | |
| 700 | 1 | |a Yang, Huang-Yu |4 aut | |
| 700 | 1 | |a Chen, Leslie Y |4 aut | |
| 700 | 1 | |a Chiu, Yen-Ling |4 aut | |
| 700 | 1 | |a Yu, Hsiao-Yun |4 aut | |
| 700 | 1 | |a Chiu, Yi-Chieh |4 aut | |
| 700 | 1 | |a Ng, Chao-Yu |4 aut | |
| 700 | 1 | |a Chang, Ya-Ching |4 aut | |
| 700 | 1 | |a Hui, Chung-Yee R |4 aut | |
| 700 | 1 | |a Huang, Yhu-Chering |4 aut | |
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