Nitric Oxide controls shoot meristem activity via regulation of DNA methylation
Abstract Despite the importance of Nitric Oxide (NO) as signaling molecule in both plant and animal development1–7, the regulatory mechanisms downstream of NO remain largely unclear. Here, we show that NO is involved inArabidopsisshoot stem cell control via modifying expression and activity ofARGONAUTE 4(AGO4), a core component of the RNA directed DNA Methylation (RdDM) pathway. Mutations in components of the RdDM pathway cause meristematic defects, and reduce responses of the stem cell system to NO signaling. Importantly, we found that the stem cell inducing WUSCHEL transcription factor directly interacts with AGO4 in an NO dependent manner, explaining how these two signaling systems may converge to modify DNA methylation patterns. Taken together, our results reveal that NO signaling plays an important role in controlling plant stem cell homeostasis via the regulation ofde novoDNA methylation..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 23. Apr. Zur Gesamtaufnahme - year:2024 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zeng, Jian [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
|---|
| doi: |
10.1101/2023.10.06.561157 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI04110983X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI04110983X | ||
| 003 | DE-627 | ||
| 005 | 20240425104439.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231011s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2023.10.06.561157 |2 doi | |
| 035 | |a (DE-627)XBI04110983X | ||
| 035 | |a (biorXiv)10.1101/2023.10.06.561157 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zeng, Jian |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Nitric Oxide controls shoot meristem activity via regulation of DNA methylation |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Despite the importance of Nitric Oxide (NO) as signaling molecule in both plant and animal development1–7, the regulatory mechanisms downstream of NO remain largely unclear. Here, we show that NO is involved inArabidopsisshoot stem cell control via modifying expression and activity ofARGONAUTE 4(AGO4), a core component of the RNA directed DNA Methylation (RdDM) pathway. Mutations in components of the RdDM pathway cause meristematic defects, and reduce responses of the stem cell system to NO signaling. Importantly, we found that the stem cell inducing WUSCHEL transcription factor directly interacts with AGO4 in an NO dependent manner, explaining how these two signaling systems may converge to modify DNA methylation patterns. Taken together, our results reveal that NO signaling plays an important role in controlling plant stem cell homeostasis via the regulation ofde novoDNA methylation. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Zhao, Xin’Ai |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Zhe |e verfasserin |4 aut | |
| 700 | 1 | |a Hidalgo, Inés |e verfasserin |4 aut | |
| 700 | 1 | |a Gebert, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Pengfei |e verfasserin |4 aut | |
| 700 | 1 | |a Wenzl, Christian |e verfasserin |4 aut | |
| 700 | 1 | |a Gornik, Sebastian G. |e verfasserin |4 aut | |
| 700 | 1 | |a Lohmann, Jan U. |e verfasserin |0 (orcid)0000-0003-3667-187X |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2024) vom: 23. Apr. |
| 773 | 1 | 8 | |g year:2024 |g day:23 |g month:04 |
| 856 | 4 | 0 | |u https://doi.org/10.1038/s41467-023-43705-1 |x 0 |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2023.10.06.561157 |x 0 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2024 |b 23 |c 04 | ||