Genome-wide Characterization of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi
Abstract Bacteriophages constitute one of the largest sources of unknown gene content in the biosphere. Even for well-studied model phages, robust experimental approaches to identify and study their essential genes remain elusive. We uncover and exploit the conserved vulnerability of the phage transcriptome to facilitate genome-wide protein expression knockdown via programmable RNA-binding protein dRfxCas13d (CRISPRi-ART) across diverse phages and their host. Establishing the first broad-spectrum phage functional genomics platform, we predict over 90 essential genes across four phage genomes, a third of which have no known function. These results highlight hidden infection strategies encoded in the most abundant biological entities on earth and provide a facile platform to study them..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 21. Sept. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Adler, Benjamin A. [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2023.09.18.558157 |
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| PPN (Katalog-ID): |
XBI040904822 |
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| 100 | 1 | |a Adler, Benjamin A. |e verfasserin |0 (orcid)0000-0002-7488-3040 |4 aut | |
| 245 | 1 | 0 | |a Genome-wide Characterization of Diverse Bacteriophages Enabled by RNA-Binding CRISPRi |
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| 520 | |a Abstract Bacteriophages constitute one of the largest sources of unknown gene content in the biosphere. Even for well-studied model phages, robust experimental approaches to identify and study their essential genes remain elusive. We uncover and exploit the conserved vulnerability of the phage transcriptome to facilitate genome-wide protein expression knockdown via programmable RNA-binding protein dRfxCas13d (CRISPRi-ART) across diverse phages and their host. Establishing the first broad-spectrum phage functional genomics platform, we predict over 90 essential genes across four phage genomes, a third of which have no known function. These results highlight hidden infection strategies encoded in the most abundant biological entities on earth and provide a facile platform to study them. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Al-Shimary, Muntathar J. |0 (orcid)0000-0002-7021-2565 |4 aut | |
| 700 | 1 | |a Patel, Jaymin R. |4 aut | |
| 700 | 1 | |a Armbruster, Emily |4 aut | |
| 700 | 1 | |a Colognori, David |0 (orcid)0000-0001-6995-7079 |4 aut | |
| 700 | 1 | |a Charles, Emeric J. |0 (orcid)0000-0002-3465-5625 |4 aut | |
| 700 | 1 | |a Miller, Kate V. |4 aut | |
| 700 | 1 | |a Lahiri, Arushi |0 (orcid)0000-0001-6834-4692 |4 aut | |
| 700 | 1 | |a Trinidad, Marena |0 (orcid)0000-0001-7839-4642 |4 aut | |
| 700 | 1 | |a Boger, Ron |0 (orcid)0000-0002-4467-271X |4 aut | |
| 700 | 1 | |a Nomburg, Jason |0 (orcid)0000-0001-7807-8658 |4 aut | |
| 700 | 1 | |a Beurnier, Sebastien |0 (orcid)0000-0003-1533-8787 |4 aut | |
| 700 | 1 | |a Cui, Michael L. |0 (orcid)0000-0002-4352-9831 |4 aut | |
| 700 | 1 | |a Barrangou, Rodolphe |4 aut | |
| 700 | 1 | |a Mutalik, Vivek K. |0 (orcid)0000-0001-7934-0400 |4 aut | |
| 700 | 1 | |a Schoeniger, Joseph S. |0 (orcid)0000-0002-2719-7435 |4 aut | |
| 700 | 1 | |a Pogliano, Joseph A. |0 (orcid)0000-0001-8989-2655 |4 aut | |
| 700 | 1 | |a Savage, David F. |0 (orcid)0000-0003-0042-2257 |4 aut | |
| 700 | 1 | |a Doudna, Jennifer A. |0 (orcid)0000-0001-9161-999X |4 aut | |
| 700 | 1 | |a Cress, Brady F. |0 (orcid)0000-0002-2948-2846 |4 aut | |
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