Dietary road salt and monarch butterflies: minimal effects on larval growth, immunity, wing coloration, and migration to Mexico
Abstract The spectacular migration of the monarch butterfly is under threat from the loss of habitat and the decline of their milkweed host plants. In the northern part of their range, roadsides could potentially produce millions of monarchs annually due to high densities of milkweed, however roadside milkweed can accumulate chemicals from roads, such as sodium from road salt. Controlled lab studies have shown mixed effects of sodium on monarch development: small increases can be beneficial as sodium is an important micronutrient in brain and muscle development, but large increases can sometimes decrease survival. It is unclear how dietary sodium affects performance in ecologically relevant conditions, and the migration itself. In this experiment, we raised monarchs outdoors, in migration-inducing conditions, on milkweed sprayed with three levels of sodium chloride. We released 2500 tagged monarchs and held an additional 250 for further lab assays. While our recovery rates to the wintering grounds were low (N = 7 individuals), individuals from all three sodium chloride treatments made it to Mexico. Butterflies reared on control milkweed and low salt concentrated sodium in their tissues, while those on high salt diets excreted sodium, suggesting levels were above a physiological optimum. There were no effects of treatment on wing coloration, survival, body size, immunity, or parasite prevalence. Taken together, our results suggest that monarchs are robust to levels of sodium in milkweeds found along roadsides, which is promising with respect to monarch conservation efforts that promote roadside habitat.<jats:sec id="s21">Significance Statement Monarch butterflies are a flagship species for pollinator conservation, and were recently being listed as endangered by the IUCN. Roadside habitat is a target for monarch breeding habitat as they often have high densities of milkweed, the monarch hostplant. However, roadsides can also have high levels of pollutants, such as salt from deicing treatments. We reared monarch caterpillars on sodium treated milkweeds, measuring a suite of performance measures, and releasing nearly 2500 tagged monarchs for migration. We found little effect of salt on migration to Mexico, survival, body size, development time, parasite prevalence, immunity, or coloration. Monarchs appear robust to levels of sodium found in milkweed along roadsides, supporting the possibility of roadsides as habitat..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 08. Sept. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hund, Amanda K [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2023.09.04.554310 |
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| PPN (Katalog-ID): |
XBI040743543 |
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| 100 | 1 | |a Hund, Amanda K |e verfasserin |0 (orcid)0000-0001-7758-6757 |4 aut | |
| 245 | 1 | 0 | |a Dietary road salt and monarch butterflies: minimal effects on larval growth, immunity, wing coloration, and migration to Mexico |
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| 520 | |a Abstract The spectacular migration of the monarch butterfly is under threat from the loss of habitat and the decline of their milkweed host plants. In the northern part of their range, roadsides could potentially produce millions of monarchs annually due to high densities of milkweed, however roadside milkweed can accumulate chemicals from roads, such as sodium from road salt. Controlled lab studies have shown mixed effects of sodium on monarch development: small increases can be beneficial as sodium is an important micronutrient in brain and muscle development, but large increases can sometimes decrease survival. It is unclear how dietary sodium affects performance in ecologically relevant conditions, and the migration itself. In this experiment, we raised monarchs outdoors, in migration-inducing conditions, on milkweed sprayed with three levels of sodium chloride. We released 2500 tagged monarchs and held an additional 250 for further lab assays. While our recovery rates to the wintering grounds were low (N = 7 individuals), individuals from all three sodium chloride treatments made it to Mexico. Butterflies reared on control milkweed and low salt concentrated sodium in their tissues, while those on high salt diets excreted sodium, suggesting levels were above a physiological optimum. There were no effects of treatment on wing coloration, survival, body size, immunity, or parasite prevalence. Taken together, our results suggest that monarchs are robust to levels of sodium in milkweeds found along roadsides, which is promising with respect to monarch conservation efforts that promote roadside habitat.<jats:sec id="s21">Significance Statement Monarch butterflies are a flagship species for pollinator conservation, and were recently being listed as endangered by the IUCN. Roadside habitat is a target for monarch breeding habitat as they often have high densities of milkweed, the monarch hostplant. However, roadsides can also have high levels of pollutants, such as salt from deicing treatments. We reared monarch caterpillars on sodium treated milkweeds, measuring a suite of performance measures, and releasing nearly 2500 tagged monarchs for migration. We found little effect of salt on migration to Mexico, survival, body size, development time, parasite prevalence, immunity, or coloration. Monarchs appear robust to levels of sodium found in milkweed along roadsides, supporting the possibility of roadsides as habitat. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Mitchell, Timothy S. |4 aut | |
| 700 | 1 | |a Ramirez, Isabel |4 aut | |
| 700 | 1 | |a Zambre, Amod |0 (orcid)0000-0002-0054-5532 |4 aut | |
| 700 | 1 | |a Hagg, Lili |4 aut | |
| 700 | 1 | |a Stene, Anne |4 aut | |
| 700 | 1 | |a Porter, Karilyn |4 aut | |
| 700 | 1 | |a Carper, Adrian |0 (orcid)0000-0002-8657-3613 |4 aut | |
| 700 | 1 | |a Agnew, Lauren |4 aut | |
| 700 | 1 | |a Shephard, Alex |4 aut | |
| 700 | 1 | |a Kobiela, Megan |4 aut | |
| 700 | 1 | |a Oberhauser, Karen |4 aut | |
| 700 | 1 | |a Taylor, Orley R. |0 (orcid)0000-0002-1401-8282 |4 aut | |
| 700 | 1 | |a Snell-Rood, Emilie |0 (orcid)0000-0002-2972-5895 |4 aut | |
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