Nanoscale analysis of human G1 and metaphase chromatin in situ
ABSTRACT The structure of chromatin at the nucleosome level inside cells is mysterious. Here we presentin situcryo-ET analyses of chromatin in both G1 and metaphase RPE-1 cells. G1 nucleosomes are concentrated in globular chromatin domains and metaphase nucleosomes are concentrated in the chromatids. Classification analysis reveals that canonical mononucleosomes, ordered stacked dinucleosomes, and mononucleosomes with a disordered gyre-proximal density are abundant in both cell-cycle states. Class averages that have more than two stacked nucleosomes or that have side-by-side dinucleosomes are not detected, suggesting that groups of more than two nucleosomes are heterogeneous. Large multi-megadalton structures are abundant in G1 nucleoplasm, but not found in G1 chromatin domains and metaphase chromatin. The macromolecular phenotypes studied here represent a starting point for the comparative analysis of condensation in normal and unhealthy human cells, in other cell-cycle states, other organisms, andin vitrochromatin assemblies..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2023 |
|---|---|
| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 02. Aug. Zur Gesamtaufnahme - year:2023 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Chen, Jon Ken [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| doi: |
10.1101/2023.07.31.551204 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI04037842X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI04037842X | ||
| 003 | DE-627 | ||
| 005 | 20231205144523.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230801s2023 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2023.07.31.551204 |2 doi | |
| 035 | |a (DE-627)XBI04037842X | ||
| 035 | |a (biorXiv)10.1101/2023.07.31.551204 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Chen, Jon Ken |e verfasserin |0 (orcid)0000-0002-3837-5444 |4 aut | |
| 245 | 1 | 0 | |a Nanoscale analysis of human G1 and metaphase chromatin in situ |
| 264 | 1 | |c 2023 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a ABSTRACT The structure of chromatin at the nucleosome level inside cells is mysterious. Here we presentin situcryo-ET analyses of chromatin in both G1 and metaphase RPE-1 cells. G1 nucleosomes are concentrated in globular chromatin domains and metaphase nucleosomes are concentrated in the chromatids. Classification analysis reveals that canonical mononucleosomes, ordered stacked dinucleosomes, and mononucleosomes with a disordered gyre-proximal density are abundant in both cell-cycle states. Class averages that have more than two stacked nucleosomes or that have side-by-side dinucleosomes are not detected, suggesting that groups of more than two nucleosomes are heterogeneous. Large multi-megadalton structures are abundant in G1 nucleoplasm, but not found in G1 chromatin domains and metaphase chromatin. The macromolecular phenotypes studied here represent a starting point for the comparative analysis of condensation in normal and unhealthy human cells, in other cell-cycle states, other organisms, andin vitrochromatin assemblies. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Liu, Tingsheng |0 (orcid)0000-0001-8371-9827 |4 aut | |
| 700 | 1 | |a Cai, Shujun |0 (orcid)0000-0002-8558-6279 |4 aut | |
| 700 | 1 | |a Ruan, Weimei |0 (orcid)0000-0002-3459-5837 |4 aut | |
| 700 | 1 | |a Ng, Cai Tong |0 (orcid)0000-0002-1082-8667 |4 aut | |
| 700 | 1 | |a Shi, Jian |0 (orcid)0000-0003-3810-5302 |4 aut | |
| 700 | 1 | |a Surana, Uttam |0 (orcid)0000-0002-4785-6514 |4 aut | |
| 700 | 1 | |a Gan, Lu |0 (orcid)0000-0002-8685-4896 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2023) vom: 02. Aug. |
| 773 | 1 | 8 | |g year:2023 |g day:02 |g month:08 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2023.07.31.551204 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2023 |b 02 |c 08 | ||