hnRNPUL1 ensures efficient Integrator-mediated cleavage of snRNAs and is mutated in amyotrophic lateral sclerosis
Abstract Integrator cleaves nascent RNA, triggering RNA polymerase II transcription termination, but how cleavage is regulated is poorly understood. Here we show hnRNPUL1 ensures efficient Integrator-mediated cleavage of nascent RNA downstream of snRNA genes and, in the case of U2 snRNA, binds a terminal stem-loop involved in this process. In the nucleoplasm, hnRNPUL1 binds U4 snRNA and SART3 and enables efficient reformation of the U4:U6 di-snRNP for further rounds of pre-mRNA splicing. Sustained hnRNPUL1 loss leads to reduced levels of snRNAs, defects in histone mRNA 3′ end processing and loss of Cajal bodies. hnRNPUL1 binds RNA through multiple domains, including a globular central domain comprising tightly juxtaposed SPRY and dead polynucleotide kinase folds. This latter fold allows binding to 5′-monophosphorylated RNAs in a mutually exclusive manner with ATP binding and functions as an XRN2 antagonist when overexpressed. We identify a cohort of amyotrophic lateral sclerosis patients harbouring disruptive mutations in hnRNPUL1. SMN loss in spinal muscular atrophy and hnRNPUL1 loss both disrupt snRNP biogenesis, leading to motor neuron death, suggesting a common aetiology..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 26. Juli Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yonchev, Ivaylo D. [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2023.07.22.550030 |
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| PPN (Katalog-ID): |
XBI040290689 |
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| 520 | |a Abstract Integrator cleaves nascent RNA, triggering RNA polymerase II transcription termination, but how cleavage is regulated is poorly understood. Here we show hnRNPUL1 ensures efficient Integrator-mediated cleavage of nascent RNA downstream of snRNA genes and, in the case of U2 snRNA, binds a terminal stem-loop involved in this process. In the nucleoplasm, hnRNPUL1 binds U4 snRNA and SART3 and enables efficient reformation of the U4:U6 di-snRNP for further rounds of pre-mRNA splicing. Sustained hnRNPUL1 loss leads to reduced levels of snRNAs, defects in histone mRNA 3′ end processing and loss of Cajal bodies. hnRNPUL1 binds RNA through multiple domains, including a globular central domain comprising tightly juxtaposed SPRY and dead polynucleotide kinase folds. This latter fold allows binding to 5′-monophosphorylated RNAs in a mutually exclusive manner with ATP binding and functions as an XRN2 antagonist when overexpressed. We identify a cohort of amyotrophic lateral sclerosis patients harbouring disruptive mutations in hnRNPUL1. SMN loss in spinal muscular atrophy and hnRNPUL1 loss both disrupt snRNP biogenesis, leading to motor neuron death, suggesting a common aetiology. | ||
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| 700 | 1 | |a Apostol, Carmen V. |0 (orcid)0000-0002-2309-7285 |4 aut | |
| 700 | 1 | |a Griffith, Llywelyn |0 (orcid)0009-0008-0223-8895 |4 aut | |
| 700 | 1 | |a Li, Ang |4 aut | |
| 700 | 1 | |a Butler, Larissa |4 aut | |
| 700 | 1 | |a Patrick, Helen |4 aut | |
| 700 | 1 | |a Aguilar-Martinez, Elisa |4 aut | |
| 700 | 1 | |a Whelan, Ashleigh G. R. |4 aut | |
| 700 | 1 | |a Evans, Caroline |4 aut | |
| 700 | 1 | |a Dickman, Mark J. |4 aut | |
| 700 | 1 | |a Cooper-Knock, Johnathan |4 aut | |
| 700 | 1 | |a Shaw, Pamela J. |4 aut | |
| 700 | 1 | |a West, Steven |4 aut | |
| 700 | 1 | |a Barbaric, Ivana |4 aut | |
| 700 | 1 | |a Sudbery, Ian M. |4 aut | |
| 700 | 1 | |a Wilson, Stuart A. |4 aut | |
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