Recent emergence of cephalosporin resistant<i>Salmonella</i>Typhi in India due to the endemic clone acquiring IncFIB(K) plasmid encoding<i>bla</i><sub>CTX-M-15</sub>gene
Abstract The emergence and spread ofSalmonellaTyphi (S. Typhi) resistant to third generation cephalosporins are a serious global health concern. In this study, we have genomically characterized 142 cephalosporin resistantS. Typhi strains isolated from Gujarat, India. Comparative genome analysis of study isolates revealed the emergence of a new clone of ceftriaxone-resistantS. Typhi harboring three plasmids of the incompatibility group IncFIB(K), IncX1 and IncFIB(pHCM2). Among the three, IncFIB(K) plasmid confers resistance to third-generation cephalosporins by means ofblaCTX-M-15gene, as well as other resistance determinants such asaph(3″),aph(6′),sul2,dfrA14,qnrSandtetA. Phylogenetic analysis of strains revealed that the isolates from Gujarat (n=140/142) belong to a distinct subclade (genotype 4.3.1.2.2) within genotype 4.3.1.2 (H58 lineage II). SNP-based phylogenetic analysis of the core genes in IncFIB(K) suggested a close relatedness of the plasmid backbone to that of IncFIB(K) from other Enterobacteriales. This implies that H58 lineage II can acquire MDR plasmids from other Enterobacteriales provided compensatory evolution balances the associated fitness costs. Although, as previously reported, exposure to the third generation cephalosporins during treatment may have selected for these variants, this could indicate the potential onset of a new wave of ceftriaxone-resistantS. Typhi in India. The implementation of control measures such as vaccination, improved water, sanitation, etc., could be undertaken in areas where MDR or XDRS. Typhi strains are prevalent..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 27. Apr. Zur Gesamtaufnahme - year:2024 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Priya T, Tharani [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| doi: |
10.1101/2023.07.05.547856 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI040126234 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI040126234 | ||
| 003 | DE-627 | ||
| 005 | 20240428100914.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230707s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2023.07.05.547856 |2 doi | |
| 035 | |a (DE-627)XBI040126234 | ||
| 035 | |a (biorXiv)10.1101/2023.07.05.547856 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Priya T, Tharani |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Recent emergence of cephalosporin resistant<i>Salmonella</i>Typhi in India due to the endemic clone acquiring IncFIB(K) plasmid encoding<i>bla</i><sub>CTX-M-15</sub>gene |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract The emergence and spread ofSalmonellaTyphi (S. Typhi) resistant to third generation cephalosporins are a serious global health concern. In this study, we have genomically characterized 142 cephalosporin resistantS. Typhi strains isolated from Gujarat, India. Comparative genome analysis of study isolates revealed the emergence of a new clone of ceftriaxone-resistantS. Typhi harboring three plasmids of the incompatibility group IncFIB(K), IncX1 and IncFIB(pHCM2). Among the three, IncFIB(K) plasmid confers resistance to third-generation cephalosporins by means ofblaCTX-M-15gene, as well as other resistance determinants such asaph(3″),aph(6′),sul2,dfrA14,qnrSandtetA. Phylogenetic analysis of strains revealed that the isolates from Gujarat (n=140/142) belong to a distinct subclade (genotype 4.3.1.2.2) within genotype 4.3.1.2 (H58 lineage II). SNP-based phylogenetic analysis of the core genes in IncFIB(K) suggested a close relatedness of the plasmid backbone to that of IncFIB(K) from other Enterobacteriales. This implies that H58 lineage II can acquire MDR plasmids from other Enterobacteriales provided compensatory evolution balances the associated fitness costs. Although, as previously reported, exposure to the third generation cephalosporins during treatment may have selected for these variants, this could indicate the potential onset of a new wave of ceftriaxone-resistantS. Typhi in India. The implementation of control measures such as vaccination, improved water, sanitation, etc., could be undertaken in areas where MDR or XDRS. Typhi strains are prevalent. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Jacob, Jobin John |e verfasserin |4 aut | |
| 700 | 1 | |a Aravind, V |e verfasserin |4 aut | |
| 700 | 1 | |a Monisha Priya, T |e verfasserin |4 aut | |
| 700 | 1 | |a Shah, Bhavini |e verfasserin |4 aut | |
| 700 | 1 | |a Iyer, Veena |e verfasserin |4 aut | |
| 700 | 1 | |a Maheshwari, Geeti |e verfasserin |4 aut | |
| 700 | 1 | |a Trivedi, Urmi |e verfasserin |4 aut | |
| 700 | 1 | |a Shah, Anand |e verfasserin |4 aut | |
| 700 | 1 | |a Patel, Pooja |e verfasserin |4 aut | |
| 700 | 1 | |a Gaigawale, Anushree |e verfasserin |4 aut | |
| 700 | 1 | |a Yesudoss, M |e verfasserin |4 aut | |
| 700 | 1 | |a Narayanan, Pavithra Sathya |e verfasserin |4 aut | |
| 700 | 1 | |a Mutreja, Ankur |e verfasserin |4 aut | |
| 700 | 1 | |a Carey, Megan |e verfasserin |4 aut | |
| 700 | 1 | |a John, Jacob |e verfasserin |4 aut | |
| 700 | 1 | |a Kang, Gagandeep |e verfasserin |4 aut | |
| 700 | 1 | |a Veeraraghavan, Balaji |e verfasserin |0 (orcid)0000-0002-8662-4257 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2024) vom: 27. Apr. |
| 773 | 1 | 8 | |g year:2024 |g day:27 |g month:04 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2023.07.05.547856 |x 0 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2024 |b 27 |c 04 | ||