Meta-omics analysis revealed structural and metabolic shifts of acidification communities after feeding with thermal hydrolysis pretreated food waste
Abstract In this study, a reduced total volatile fatty acid (VFA) yield (VFA/tCOD,P= .003) and accumulated fractions of butyric acid (BA) (BA/total VFA,P< 0.001) was observed after feeding the arrested anaerobic digestor with thermal hydrolysis pretreated food waste. Further analysis through meta-omics revealed a reduction in the diversity of acidification communities (Shannon index,P= .03), indicating a sterilization effect of THP on the indigenous microbes in the feedstock. Additionally, although both the community-level acidification genetic potential and transcriptional activities were reduced (DNA and mRNA RPKM log2 fold change = 1.77 and 0.11, respectively) after the inclusion of THP, the remaining microbial communities exhibited an elevated mRNA/DNA RPKM ratio in acidification-related genes, especially within the AA and BA metabolic networks. This suggested that the elevated BA fraction might stem from improved chain-elongation activities using AA as a precursor. Furthermore, the inclusion of THP in the feedstock changed the main functional microbial groups of BA production (via the genecrt-buk2) fromSelenomonasandBacteroidestoPrevotellaandMegasphaera. Overall, these results reveal a complex shift in the acidification community and provide new insights to evaluate THP technologies behind the formed black box of VFA production performance..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 27. Mai Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jiang, Minxi [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2023.05.23.541846 |
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| PPN (Katalog-ID): |
XBI039692183 |
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| 100 | 1 | |a Jiang, Minxi |e verfasserin |0 (orcid)0000-0002-0796-2590 |4 aut | |
| 245 | 1 | 0 | |a Meta-omics analysis revealed structural and metabolic shifts of acidification communities after feeding with thermal hydrolysis pretreated food waste |
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| 520 | |a Abstract In this study, a reduced total volatile fatty acid (VFA) yield (VFA/tCOD,P= .003) and accumulated fractions of butyric acid (BA) (BA/total VFA,P< 0.001) was observed after feeding the arrested anaerobic digestor with thermal hydrolysis pretreated food waste. Further analysis through meta-omics revealed a reduction in the diversity of acidification communities (Shannon index,P= .03), indicating a sterilization effect of THP on the indigenous microbes in the feedstock. Additionally, although both the community-level acidification genetic potential and transcriptional activities were reduced (DNA and mRNA RPKM log2 fold change = 1.77 and 0.11, respectively) after the inclusion of THP, the remaining microbial communities exhibited an elevated mRNA/DNA RPKM ratio in acidification-related genes, especially within the AA and BA metabolic networks. This suggested that the elevated BA fraction might stem from improved chain-elongation activities using AA as a precursor. Furthermore, the inclusion of THP in the feedstock changed the main functional microbial groups of BA production (via the genecrt-buk2) fromSelenomonasandBacteroidestoPrevotellaandMegasphaera. Overall, these results reveal a complex shift in the acidification community and provide new insights to evaluate THP technologies behind the formed black box of VFA production performance. | ||
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| 700 | 1 | |a Chandran, Kartik |4 aut | |
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| 700 | 1 | |a Gilmore, Kevin |4 aut | |
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