<i>In silico</i>mechanics of stem cells intramyocardially transplanted with a biomaterial injectate for treatment of myocardial infarction
Abstract Purpose Biomaterial and stem cell delivery are promising approaches to treating myocardial infarction. However, the mechanical and biochemical mechanisms underlying the therapeutic benefits require further clarification. This study aimed to assess the deformation of stem cells injected with the biomaterial into the infarcted heart.Methods A microstructural finite element model of a mid-wall infarcted myocardial region was developed fromex vivomicrocomputed tomography data of a rat heart with left ventricular infarct and intramyocardial biomaterial injectate. Nine cells were numerically seeded in the injectate of the microstructural model. The microstructural and a previously developed biventricular finite element model of the same rat heart were used to quantify the deformation of the cells during a cardiac cycle for a biomaterial elastic modulus (Einj) ranging between 4.1 and 405,900 kPa.Results The transplanted cells’ deformation was largest for Einj= 7.4 kPa, matching that of the cells, and decreased for an increase and decrease in Einj. The cell deformation was more sensitive to Einjchanges for softer (Einj≤ 738 kPa) than stiffer biomaterials.Conclusions Combining the microstructural and biventricular finite element models enables quantifying micromechanics and signalling of transplanted cells in the heart. The approach offers a broader scope forin silicoinvestigations of biomaterial and cell therapies for myocardial infarction and other cardiac pathologies..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 28. März Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Motchon, YD [VerfasserIn] |
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Volltext [kostenfrei] |
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| doi: |
10.1101/2023.05.10.540185 |
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| PPN (Katalog-ID): |
XBI039565564 |
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| 520 | |a Abstract Purpose Biomaterial and stem cell delivery are promising approaches to treating myocardial infarction. However, the mechanical and biochemical mechanisms underlying the therapeutic benefits require further clarification. This study aimed to assess the deformation of stem cells injected with the biomaterial into the infarcted heart.Methods A microstructural finite element model of a mid-wall infarcted myocardial region was developed fromex vivomicrocomputed tomography data of a rat heart with left ventricular infarct and intramyocardial biomaterial injectate. Nine cells were numerically seeded in the injectate of the microstructural model. The microstructural and a previously developed biventricular finite element model of the same rat heart were used to quantify the deformation of the cells during a cardiac cycle for a biomaterial elastic modulus (Einj) ranging between 4.1 and 405,900 kPa.Results The transplanted cells’ deformation was largest for Einj= 7.4 kPa, matching that of the cells, and decreased for an increase and decrease in Einj. The cell deformation was more sensitive to Einjchanges for softer (Einj≤ 738 kPa) than stiffer biomaterials.Conclusions Combining the microstructural and biventricular finite element models enables quantifying micromechanics and signalling of transplanted cells in the heart. The approach offers a broader scope forin silicoinvestigations of biomaterial and cell therapies for myocardial infarction and other cardiac pathologies. | ||
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| 700 | 1 | |a Abdalrahman, T |0 (orcid)0000-0002-0320-811X |4 aut | |
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