Structure of the human Bre1 complex bound to the nucleosome
Summary Histone H2B monoubiquitination (at Lys120 in humans) regulates transcription elongation and DNA repair. In humans, H2B monoubiquitination is catalyzed by the heterodimeric Bre1 complex composed of Bre1A/RNF20 and Bre1B/RNF40. The Bre1 proteins generally function as tumor suppressors, while in certain cancers, they facilitate cancer cell proliferation. To reveal the structural basis of H2BK120 ubiquitination and its regulation, we determined the cryo-EM structure of the human Bre1 complex bound to the nucleosome. The two RING domains of Bre1A and Bre1B recognize the acidic patch and the nucleosomal DNA phosphates around SHL 6.0, which are ideally located to recruit the E2 enzyme and ubiquitin for H2BK120-specific ubiquitination. Mutational experiments suggest that the two RING domains bind in both orientations and that ubiquitination occurs when Bre1A binds to the acidic patch. Our results provide insights into the relationships between Bre1 proteins and cancer and suggest that H2B monoubiquitination can be regulated by nuclesomal DNA flexibility..
Medienart: |
Preprint |
---|
Erscheinungsjahr: |
2024 |
---|---|
Erschienen: |
2024 |
Enthalten in: |
bioRxiv.org - (2024) vom: 29. Apr. Zur Gesamtaufnahme - year:2024 |
---|
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Onishi, Shuhei [VerfasserIn] |
---|
Links: |
---|
Themen: |
---|
doi: |
10.1101/2023.03.31.535082 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
XBI039138879 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | XBI039138879 | ||
003 | DE-627 | ||
005 | 20240430104626.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230403s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1101/2023.03.31.535082 |2 doi | |
035 | |a (DE-627)XBI039138879 | ||
035 | |a (biorXiv)10.1101/2023.03.31.535082 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Onishi, Shuhei |e verfasserin |0 (orcid)0000-0002-4623-6276 |4 aut | |
245 | 1 | 0 | |a Structure of the human Bre1 complex bound to the nucleosome |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Summary Histone H2B monoubiquitination (at Lys120 in humans) regulates transcription elongation and DNA repair. In humans, H2B monoubiquitination is catalyzed by the heterodimeric Bre1 complex composed of Bre1A/RNF20 and Bre1B/RNF40. The Bre1 proteins generally function as tumor suppressors, while in certain cancers, they facilitate cancer cell proliferation. To reveal the structural basis of H2BK120 ubiquitination and its regulation, we determined the cryo-EM structure of the human Bre1 complex bound to the nucleosome. The two RING domains of Bre1A and Bre1B recognize the acidic patch and the nucleosomal DNA phosphates around SHL 6.0, which are ideally located to recruit the E2 enzyme and ubiquitin for H2BK120-specific ubiquitination. Mutational experiments suggest that the two RING domains bind in both orientations and that ubiquitination occurs when Bre1A binds to the acidic patch. Our results provide insights into the relationships between Bre1 proteins and cancer and suggest that H2B monoubiquitination can be regulated by nuclesomal DNA flexibility. | ||
650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
700 | 1 | |a Uchiyama, Kotone |e verfasserin |4 aut | |
700 | 1 | |a Sato, Ko |e verfasserin |4 aut | |
700 | 1 | |a Okada, Chikako |e verfasserin |4 aut | |
700 | 1 | |a Kobayashi, Shunsuke |e verfasserin |4 aut | |
700 | 1 | |a Nishizawa, Tomohiro |e verfasserin |0 (orcid)0000-0001-7463-8398 |4 aut | |
700 | 1 | |a Nureki, Osamu |e verfasserin |0 (orcid)0000-0003-1813-7008 |4 aut | |
700 | 1 | |a Ogata, Kazuhiro |e verfasserin |0 (orcid)0000-0003-2952-3027 |4 aut | |
700 | 1 | |a Sengoku, Toru |e verfasserin |0 (orcid)0000-0001-9461-8714 |4 aut | |
773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2024) vom: 29. Apr. |
773 | 1 | 8 | |g year:2024 |g day:29 |g month:04 |
856 | 4 | 0 | |u https://doi.org/10.1038/s41467-024-46910-8 |x 0 |z lizenzpflichtig |3 Volltext |
856 | 4 | 0 | |u http://dx.doi.org/10.1101/2023.03.31.535082 |x 0 |z kostenfrei |3 Volltext |
912 | |a GBV_XBI | ||
951 | |a AR | ||
952 | |j 2024 |b 29 |c 04 |