Oct4 redox sensitivity potentiates reprogramming and differentiation
Abstract The transcription factor Oct4/Pou5f1 is a component of the regulatory circuitry governing pluripotency and is widely used to induce pluripotency from somatic cells. Here we use domain swapping and mutagenesis to study Oct4’s reprogramming ability, identifying a redox-sensitive DNA binding domain cysteine residue (Cys48) as a key determinant of reprogramming and differentiation. Oct4 Cys48 sensitizes the protein to oxidative inhibition of DNA binding activity and promotes oxidation-mediated protein ubiquitylation.Pou5f1C48Spoint mutation has little effect on undifferentiated embryonic stem cells (ESCs), but upon retinoic acid (RA) treatment causes retention of Oct4 expression, deregulated gene expression and aberrant differentiation.Pou5f1C48SESCs also form less differentiated teratomas and contribute poorly to adult somatic tissues. Finally, we describePou5f1C48S(Janky) mice, which in the homozygous condition are severely developmentally restricted after E4.5. Rare animals bypassing this restriction appear normal at birth but are sterile. Collectively, these findings uncover a novel Oct4 redox mechanism involved in both entry into and exit from pluripotency..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2024 |
|---|---|
| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 16. Apr. Zur Gesamtaufnahme - year:2024 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Shen, Zuolian [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| Themen: |
|---|
| doi: |
10.1101/2023.02.21.529404 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI038751437 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI038751437 | ||
| 003 | DE-627 | ||
| 005 | 20240419090807.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230222s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2023.02.21.529404 |2 doi | |
| 035 | |a (DE-627)XBI038751437 | ||
| 035 | |a (biorXiv)10.1101/2023.02.21.529404 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Shen, Zuolian |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Oct4 redox sensitivity potentiates reprogramming and differentiation |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract The transcription factor Oct4/Pou5f1 is a component of the regulatory circuitry governing pluripotency and is widely used to induce pluripotency from somatic cells. Here we use domain swapping and mutagenesis to study Oct4’s reprogramming ability, identifying a redox-sensitive DNA binding domain cysteine residue (Cys48) as a key determinant of reprogramming and differentiation. Oct4 Cys48 sensitizes the protein to oxidative inhibition of DNA binding activity and promotes oxidation-mediated protein ubiquitylation.Pou5f1C48Spoint mutation has little effect on undifferentiated embryonic stem cells (ESCs), but upon retinoic acid (RA) treatment causes retention of Oct4 expression, deregulated gene expression and aberrant differentiation.Pou5f1C48SESCs also form less differentiated teratomas and contribute poorly to adult somatic tissues. Finally, we describePou5f1C48S(Janky) mice, which in the homozygous condition are severely developmentally restricted after E4.5. Rare animals bypassing this restriction appear normal at birth but are sterile. Collectively, these findings uncover a novel Oct4 redox mechanism involved in both entry into and exit from pluripotency. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
| 650 | 4 | |a 570 |7 (dpeaa)DE-84 | |
| 700 | 1 | |a Wu, Yifan |e verfasserin |4 aut | |
| 700 | 1 | |a Manna, Asit |e verfasserin |4 aut | |
| 700 | 1 | |a Yi, Chongil |e verfasserin |4 aut | |
| 700 | 1 | |a Cairns, Bradley R. |e verfasserin |0 (orcid)0000-0002-9864-8811 |4 aut | |
| 700 | 1 | |a Evason, Kimberley J. |e verfasserin |4 aut | |
| 700 | 1 | |a Chandrasekharan, Mahesh B. |e verfasserin |0 (orcid)0000-0002-9956-8354 |4 aut | |
| 700 | 1 | |a Tantin, Dean |e verfasserin |0 (orcid)0000-0003-1354-8385 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2024) vom: 16. Apr. |
| 773 | 1 | 8 | |g year:2024 |g day:16 |g month:04 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2023.02.21.529404 |x 0 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 951 | |a AR | ||
| 952 | |j 2024 |b 16 |c 04 | ||