HYENA detects oncogenes activated by distal enhancers in cancer
Abstract Somatic structural variations (SVs) in cancer can shuffle DNA content in the genome, relocate regulatory elements, and alter genome organization. Enhancer hijacking occurs when SVs relocate distal enhancers to activate proto-oncogenes. However, most enhancer hijacking studies have only focused on protein-coding genes. Here, we develop a computational algorithm “HYENA” to identify candidate oncogenes (both protein-coding and non-coding) activated by enhancer hijacking based on tumor whole-genome and transcriptome sequencing data. HYENA detects genes whose elevated expression is associated with somatic SVs by using a rank-based regression model. We systematically analyze 1,146 tumors across 25 types of adult tumors and identify a total of 108 candidate oncogenes including many non-coding genes. A long non-coding RNATOB1-AS1is activated by various types of SVs in 10% of pancreatic cancers through altered 3-dimensional genome structure. We find that high expression ofTOB1-AS1can promote cell invasion and metastasis. Our study highlights the contribution of genetic alterations in non-coding regions to tumorigenesis and tumor progression..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 16. Apr. Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Anqi [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2023.01.09.523321 |
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| PPN (Katalog-ID): |
XBI038382342 |
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| 520 | |a Abstract Somatic structural variations (SVs) in cancer can shuffle DNA content in the genome, relocate regulatory elements, and alter genome organization. Enhancer hijacking occurs when SVs relocate distal enhancers to activate proto-oncogenes. However, most enhancer hijacking studies have only focused on protein-coding genes. Here, we develop a computational algorithm “HYENA” to identify candidate oncogenes (both protein-coding and non-coding) activated by enhancer hijacking based on tumor whole-genome and transcriptome sequencing data. HYENA detects genes whose elevated expression is associated with somatic SVs by using a rank-based regression model. We systematically analyze 1,146 tumors across 25 types of adult tumors and identify a total of 108 candidate oncogenes including many non-coding genes. A long non-coding RNATOB1-AS1is activated by various types of SVs in 10% of pancreatic cancers through altered 3-dimensional genome structure. We find that high expression ofTOB1-AS1can promote cell invasion and metastasis. Our study highlights the contribution of genetic alterations in non-coding regions to tumorigenesis and tumor progression. | ||
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| 700 | 1 | |a Yesilkanal, Ali E. |e verfasserin |4 aut | |
| 700 | 1 | |a Thakur, Ashish |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Phillips, William |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Xiaoyang |e verfasserin |4 aut | |
| 700 | 1 | |a Muir, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a He, Xin |e verfasserin |4 aut | |
| 700 | 1 | |a Spitz, Francois |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Lixing |e verfasserin |4 aut | |
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