Co-occurrence and cooperation between comammox and anammox bacteria in a full-scale attached growth municipal wastewater treatment process
ABSTRACT Cooperation between comammox and anammox bacteria for nitrogen removal has been recently reported in laboratory-scale systems including synthetic community construct; however, there are no reports of full-scale municipal wastewater treatment systems with such cooperation. Here, we report intrinsic and extant kinetics as well as genome-resolved community characterization of a full-scale integrated fixed film activated sludge (IFAS) system where comammox and anammox bacteria co-occur and appear to drive nitrogen loss. Intrinsic batch kinetic assays indicated that majority of the aerobic ammonia oxidation was driven by comammox bacteria (1.75 ± 0.08 mg-N/g TS-h) in the attached growth phase with minimal contribution by ammonia oxidizing bacteria. Interestingly, a portion of total inorganic nitrogen (∼8%) was consistently lost during these aerobic assays. Aerobic nitrite oxidation assays eliminated the possibility of denitrification as a cause of nitrogen loss, while anaerobic ammonia oxidation assays resulted in rates consistent with anammox stoichiometry. Full-scale experiments at different dissolved oxygen (DO = 2-6 mg/L) set points indicated persistent nitrogen loss that was partly sensitive to DO concentrations. Genome-resolved metagenomics confirmed high abundance (relative abundance 6.53 ± 0.34%) of twoBrocadia-like anammox populations while comammox bacteria within theCa. Nitrospira nitrosa cluster were lower in abundance (0.37% ± 0.03%) andNitrosomonas-like ammonia oxidizers even lower (0.12% ± 0.02%). Collectively, our study reports for the first time the co-occurrence and co-operation of comammox and anammox bacteria in a full-scale municipal wastewater treatment system.Synopsis Comammox and anammox cooperation resulted in dissolved oxygen concentration dependent nitrogen loss in municipal wastewater treatment system..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 23. Apr. Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vilardi, Katherine [VerfasserIn] |
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| doi: |
10.1101/2022.12.05.519185 |
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| PPN (Katalog-ID): |
XBI038107244 |
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| 245 | 1 | 0 | |a Co-occurrence and cooperation between comammox and anammox bacteria in a full-scale attached growth municipal wastewater treatment process |
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| 520 | |a ABSTRACT Cooperation between comammox and anammox bacteria for nitrogen removal has been recently reported in laboratory-scale systems including synthetic community construct; however, there are no reports of full-scale municipal wastewater treatment systems with such cooperation. Here, we report intrinsic and extant kinetics as well as genome-resolved community characterization of a full-scale integrated fixed film activated sludge (IFAS) system where comammox and anammox bacteria co-occur and appear to drive nitrogen loss. Intrinsic batch kinetic assays indicated that majority of the aerobic ammonia oxidation was driven by comammox bacteria (1.75 ± 0.08 mg-N/g TS-h) in the attached growth phase with minimal contribution by ammonia oxidizing bacteria. Interestingly, a portion of total inorganic nitrogen (∼8%) was consistently lost during these aerobic assays. Aerobic nitrite oxidation assays eliminated the possibility of denitrification as a cause of nitrogen loss, while anaerobic ammonia oxidation assays resulted in rates consistent with anammox stoichiometry. Full-scale experiments at different dissolved oxygen (DO = 2-6 mg/L) set points indicated persistent nitrogen loss that was partly sensitive to DO concentrations. Genome-resolved metagenomics confirmed high abundance (relative abundance 6.53 ± 0.34%) of twoBrocadia-like anammox populations while comammox bacteria within theCa. Nitrospira nitrosa cluster were lower in abundance (0.37% ± 0.03%) andNitrosomonas-like ammonia oxidizers even lower (0.12% ± 0.02%). Collectively, our study reports for the first time the co-occurrence and co-operation of comammox and anammox bacteria in a full-scale municipal wastewater treatment system.Synopsis Comammox and anammox cooperation resulted in dissolved oxygen concentration dependent nitrogen loss in municipal wastewater treatment system. | ||
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| 700 | 1 | |a Cotto, Irmarie |e verfasserin |0 (orcid)0000-0002-1626-1630 |4 aut | |
| 700 | 1 | |a Bachmann, Megan |e verfasserin |4 aut | |
| 700 | 1 | |a Parsons, Mike |e verfasserin |4 aut | |
| 700 | 1 | |a Klaus, Stephanie |e verfasserin |4 aut | |
| 700 | 1 | |a Wilson, Christopher |e verfasserin |4 aut | |
| 700 | 1 | |a Bott, Charles |e verfasserin |4 aut | |
| 700 | 1 | |a Pieper, Kelsey |e verfasserin |0 (orcid)0000-0002-0273-6527 |4 aut | |
| 700 | 1 | |a Pinto, Ameet |e verfasserin |0 (orcid)0000-0003-1089-5664 |4 aut | |
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