Bioinformatic approaches to draft the viral genome sequence of Canary Islands cases related to the multi-country Monkeypox virus 2022-outbreak
Abstract During May 2022, the World Health Organization declared community transmission of monkeypox (MPXV) due to a multi-country outbreak. In Europe, several cases of this outbreak were detected in the Canary Islands (Spain). Here we describe the combination of DNA sequencing and bioinformatic approaches, including methods forde novogenome assembly and short- and long-read technologies, used to reconstruct the first MPXV genome isolated in the Canary Islands on the 31stof May 2022 from a male adult patient with mild symptoms. We obtained the best results using a reference-based approach with short reads, evidencing 46-67 nucleotide variants against sequences from the 2018-2019 outbreak, and placing the sequence in the B.1 clade. This study demonstrates the potential of metagenomics sequencing for rapid and precise pathogen identification..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 15. Nov. Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Muñoz-Barrera, Adrián [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2022.11.08.22282035 |
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| PPN (Katalog-ID): |
XBI037857673 |
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| 245 | 1 | 0 | |a Bioinformatic approaches to draft the viral genome sequence of Canary Islands cases related to the multi-country Monkeypox virus 2022-outbreak |
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| 520 | |a Abstract During May 2022, the World Health Organization declared community transmission of monkeypox (MPXV) due to a multi-country outbreak. In Europe, several cases of this outbreak were detected in the Canary Islands (Spain). Here we describe the combination of DNA sequencing and bioinformatic approaches, including methods forde novogenome assembly and short- and long-read technologies, used to reconstruct the first MPXV genome isolated in the Canary Islands on the 31stof May 2022 from a male adult patient with mild symptoms. We obtained the best results using a reference-based approach with short reads, evidencing 46-67 nucleotide variants against sequences from the 2018-2019 outbreak, and placing the sequence in the B.1 clade. This study demonstrates the potential of metagenomics sequencing for rapid and precise pathogen identification. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Ciuffreda, Laura |e verfasserin |4 aut | |
| 700 | 1 | |a Alcoba-Florez, Julia |e verfasserin |4 aut | |
| 700 | 1 | |a Rubio-Rodríguez, Luis A. |e verfasserin |4 aut | |
| 700 | 1 | |a Rodríguez-Pérez, Héctor |e verfasserin |4 aut | |
| 700 | 1 | |a Gil-Campesino, Helena |e verfasserin |4 aut | |
| 700 | 1 | |a de Artola, Diego García-Martínez |e verfasserin |4 aut | |
| 700 | 1 | |a Salas-Hernández, Josmar |e verfasserin |4 aut | |
| 700 | 1 | |a Rodríguez-Núñez, Julia |e verfasserin |4 aut | |
| 700 | 1 | |a Íñigo-Campos, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Díez-Gil, Oscar |e verfasserin |4 aut | |
| 700 | 1 | |a González-Montelongo, Rafaela |e verfasserin |4 aut | |
| 700 | 1 | |a Valenzuela-Fernández, Agustín |e verfasserin |4 aut | |
| 700 | 1 | |a Lorenzo-Salazar, José M. |e verfasserin |4 aut | |
| 700 | 1 | |a Flores, Carlos |e verfasserin |4 aut | |
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