Emergence of ion-channel mediated electrical oscillations in<i>Escherichia coli</i>biofilms
Abstract Bacterial biofilms are communities of bacteria usually attached to solid strata and often differentiated into complex structures. Communication across biofilms has been shown to involve chemical signaling and, more recently, electrical signaling in Gram positive biofilms. We report for the first time, community-level synchronized membrane potential dynamics in three-dimensionalE. colibiofilms. Two hyperpolarization events are observed in response to light stress. The first requires mechanically sensitive ion channels (MscK, MscL and MscS) and the second needs the Kch-potassium channel. The channels mediated both local spiking of singleE. colibiofilms and long-range coordinated electrical signaling inE. colibiofilms. The electrical phenomena are explained using Hodgkin-Huxley and 3D fire-diffuse-fire agent-based models. These data demonstrate that electrical wavefronts based on potassium ions are a mechanism by which signaling occurs in Gram negative biofilms and as such may represent a conserved mechanism for communication across biofilms..
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Preprint| Erscheinungsjahr: |
2024 |
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| Erschienen: |
2024 |
| Enthalten in: |
bioRxiv.org - (2024) vom: 22. März Zur Gesamtaufnahme - year:2024 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Akabuogu, Emmanuel U. [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2022.11.09.515771 |
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| PPN (Katalog-ID): |
XBI037826522 |
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| 100 | 1 | |a Akabuogu, Emmanuel U. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Emergence of ion-channel mediated electrical oscillations in<i>Escherichia coli</i>biofilms |
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| 520 | |a Abstract Bacterial biofilms are communities of bacteria usually attached to solid strata and often differentiated into complex structures. Communication across biofilms has been shown to involve chemical signaling and, more recently, electrical signaling in Gram positive biofilms. We report for the first time, community-level synchronized membrane potential dynamics in three-dimensionalE. colibiofilms. Two hyperpolarization events are observed in response to light stress. The first requires mechanically sensitive ion channels (MscK, MscL and MscS) and the second needs the Kch-potassium channel. The channels mediated both local spiking of singleE. colibiofilms and long-range coordinated electrical signaling inE. colibiofilms. The electrical phenomena are explained using Hodgkin-Huxley and 3D fire-diffuse-fire agent-based models. These data demonstrate that electrical wavefronts based on potassium ions are a mechanism by which signaling occurs in Gram negative biofilms and as such may represent a conserved mechanism for communication across biofilms. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Martorelli, Victor |4 aut | |
| 700 | 1 | |a Krašovec, Rok |4 aut | |
| 700 | 1 | |a Roberts, Ian S. |4 aut | |
| 700 | 1 | |a Waigh, Thomas A. |4 aut | |
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